GluN1(356-385)
Based on 1 Customer Validation
GluN1 (356-385) is a polypeptide targeting NMDAR GluN1. GluN1 (356-385) induces the production of autoantibodies, which reduce the density of cell surface NMDAR clusters, impair long-term potentiation, and decrease NMDAR-mediated Ca2+ influx. As an immunogen, GluN1 (356-385) induces symptoms similar to anti-NMDAR encephalitis, including memory loss, in mice. GluN1 (356-385) can be used to establish a mouse model that mimics the pathogenesis of anti-NMDAR encephalitis. GluN1 (356-385) is applicable to research related to anti-NMDAR encephalitis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.95%
- 分子式: C154H248N46O42
- 分子量:3415.90
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保管条件:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
GluN1(356-385)-induced antibody (24 h) reduce the density of surface GluN1-positive synaptic clusters and decrease membrane GluN1 levels in primary hippocampal neurons from embryonic day 18 rats[1].
GluN1(356-385)-induced antibody (18 h at 37 °C) significantly reduces NMDAR-mediated calcium influx in primary rat hippocampal neurons on embryonic day 18[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (female, 10 weeks old, induced by active immunization with GluN1(356-385) peptide)[1]
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Dosage:200 μg
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Administration:s.c.; initial immunization on back, followed by two booster injections at 4 and 8 weeks after initial immunization
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Result:Showed significant memory loss, with decreased exploration time of a novel object and a lower discrimination index in the novel object recognition test .
Reduced visits to strangers in the three-chamber test.
Reduced hippocampal membrane GluN1 levels.
Impaired long-term potentiation (LTP) at Schaffer collateral-CA1 synapses, with a normalized field excitatory postsynaptic potential slope of 112% compared to 143% in controls.
Induced autoantibodies targeting GluN1 in cerebrospinal fluid and serum, maintained at high titers through the 12-week period, and localized primarily to the hippocampus and cerebellum.
化学情報
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性状 Solid
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分子量 3415.90
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分子式 C154H248N46O42
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Color White to off-white
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配列
Leu-Gln-Asn-Arg-Lys-Leu-Val-Gln-Val-Gly-Ile-Tyr-Asn-Gly-Thr-His-Val-Ile-Pro-Asn-Asp-Arg-Lys-Ile-Ile-Trp-Pro-Gly-Gly-Glu
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シーケンスの短縮
LQNRKLVQVGIYNGTHVIPNDRKIIWPGGE
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 66.67 mg/mL (19.52 mM; ultrasonic and adjust pH to 3 with 1 M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
0.1 M Acetic acid : 25 mg/mL (7.32 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 0.1 M Acetic acid / DMSO | 1 mM | 0.2927 mL | 1.4637 mL | 2.9275 mL | 7.3187 mL |
| 5 mM | 0.0585 mL | 0.2927 mL | 0.5855 mL | 1.4637 mL | |
| DMSO | 10 mM | 0.0293 mL | 0.1464 mL | 0.2927 mL | 0.7319 mL |
| 15 mM | 0.0195 mL | 0.0976 mL | 0.1952 mL | 0.4879 mL |
GluN1(356-385) 関連分類
HY-P5912 関連分類
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