2. Apoptosis
  3. Apoptosis
  4. Justicidin A

justicidin A is a nature product that could be isolated form Justicia procumbens. justicidin A decreases the level of Ku70 leading to translocation of Bax from the cytosol to mitochondria to induce apoptosis. justicidin A can be used in research of cancer.

For research use only. We do not sell to patients.

Justicidin A Chemical Structure

Justicidin A Chemical Structure

CAS No. : 25001-57-4

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Based on 1 publication(s) in Google Scholar

Justicidin A Related Antibodies

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Description

justicidin A is a nature product that could be isolated form Justicia procumbens. justicidin A decreases the level of Ku70 leading to translocation of Bax from the cytosol to mitochondria to induce apoptosis. justicidin A can be used in research of cancer[1].

In Vitro

justicidin A (0-2.5 μM; 6 days) inhibits cell growth in colorectal cancer cells with IC50 values of 0.110, 0.400, 0.020, 1.540 and 0.004 μM for HT-29, HCT 116, SiHa, MCF7 and T24 cells, respectively[1].
justicidin A (0.75 and 5 μM; 0-72 h) induces apoptosis in colorectal cancer cells[1].
justicidin A (0.75 and 5 μM; 0-72 h) induces the cleavage of caspases and their target proteins in human colorectal cancer cells[1].
justicidin A (0.75 and 5 μM; 0-72 h) damages mitochondria and releases cyto c and Smac from mitochondria[1].
justicidin A (0.75 and 5 μM; 0-96 h) decreases the level of Ku70 leading to translocation of Bax from the cytosol to mitochondria[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Justicidin A Related Antibodies

Cell Viability Assay[1]

Cell Line: HT-29, HCT 116, SiHa, MCF7 and T24 cells
Concentration: 0-2.5 μM
Incubation Time: 6 days
Result: Inhibited cell growth in a dose-dependent manner.

Apoptosis Analysis[1]

Cell Line: HT-29 and HCT 116 cells
Concentration: 0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time: 0, 12, 16, 20, 24, 48, and 72 hours
Result: Induced apoptosis in a time-dependent manner.
Increased the population of sub-G 1 cells in a time-dependent manner.

Western Blot Analysis[1]

Cell Line: HT-29 and HCT 116 cells
Concentration: 0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time: 0, 12, 16, 20, 24, 48, 72, and 96 hours
Result: Decreased the amount of cytosolic Ku70 in HCT 116 cells and decreased (0.6-fold) at 48 h and reached the lowest level (0.33-fold) at 96 h.

Western Blot Analysis[1]

Cell Line: HT-29 and HCT 116 cells
Concentration: 0.75 μM (HT-29 cells) and 5 μM (HCT 116 cells)
Incubation Time: 0, 12, 16, 20, 24, 48, and 72 hours
Result: Induced the cleavage of caspases and their target proteins.
In Vivo

justicidin A (6.2 mg/kg; p.o.; daily, for 56 days) suppresses the growth of human colorectal cancer cells in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NOD-SCID mice with HT-29 cells xenograftes[1]
Dosage: 6.2 mg/kg
Administration: Oral administration; daily, for 56 days
Result: Inhibited tumor growth and decreased tumor weight.
Molecular Weight

394.37

Formula

C22H18O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1OCC2=C(OC)C3=CC(OC)=C(OC)C=C3C(C4=CC=C(OCO5)C5=C4)=C12
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (42.27 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5357 mL 12.6784 mL 25.3569 mL
5 mM 0.5071 mL 2.5357 mL 5.0714 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5357 mL 12.6784 mL 25.3569 mL 63.3922 mL
5 mM 0.5071 mL 2.5357 mL 5.0714 mL 12.6784 mL
10 mM 0.2536 mL 1.2678 mL 2.5357 mL 6.3392 mL
15 mM 0.1690 mL 0.8452 mL 1.6905 mL 4.2261 mL
20 mM 0.1268 mL 0.6339 mL 1.2678 mL 3.1696 mL
25 mM 0.1014 mL 0.5071 mL 1.0143 mL 2.5357 mL
30 mM 0.0845 mL 0.4226 mL 0.8452 mL 2.1131 mL
40 mM 0.0634 mL 0.3170 mL 0.6339 mL 1.5848 mL
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Justicidin A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Justicidin A25001-57-4Apoptosisnature productJusticia procumbensKu70BaxmitochondriacancerInhibitorinhibitorinhibit

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