Cat. No.: HY-N0847

Micheliolide

8 Cited Publications

Customer Review

Based on 8 publication(s) in Google Scholar

Micheliolide is a sesquiterpene lactone with anti-cancer and anti-inflammatory effects, which is derived from Michelia compressa and Michelia champaca. Micheliolide can attenuate high glucose-stimulated NF-κB activation, IκBα degradation, and the expression of MCP-1, TGF-β1, and FN in mouse mesangial cells. Micheliolide inhibits LPS (HY-D1056)-induced activation of NF-κB and PI3K/Akt/p70S6K pathways to play an anti-inflammatory role. Micheliolide inhibits dextran sodium sulphate (DSS) (HY-116282)-induced inflammatory intestinal disease, colitis-associated cancer and rheumatic arthritis.

For research use only. We do not sell to patients.

Purity: 99.77%
CAS No.: 68370-47-8
Formula: C₁₅H₂₀O₃
Molecular Weight:248.32

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Micheliolide Chemical Structure

CAS No.: 68370-47-8

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (8)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All NF-κB Isoforms :

All PI3K Isoforms :

All Akt Isoforms :

All TGF-beta/Smad Isoforms :

Smad1
Smad3

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	5.5 μM Compound: 9, MCL	Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry Cytotoxicity against human HL60 cells after 18 hrs by annexin-V labeling-based flow cytometry	[PMID: 22985027]

In Vitro

Micheliolide (0.2-10 μM, 24 h) decreases the proliferation of mesangial cells and the production of MCP-1, TGF-β1, FN increased by a high concentration of glucose (HG) and suppresses the HG-induced degradation of IκBα^[1].
Micheliolide (1-10 μM, 18 h) does not affect the growth and apoptosis of Raw264.7. I^[2].
Micheliolide (1-10 μM, 3-18 h) inhibits LPS-triggered inflammatory responses in mouse macrophages, dendritic cells (DCs), human monocytic cell line THP-1, human CD14+ monocytes and inhibits LPS-induced NF-κB and PI3K/Akt/p70S6K activation^[2]^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[2]

Cell Line:

Mouse macrophages and dendritic cells
Concentration:

1-10 μM
Incubation Time:

3-18 h
Result:

Inhibited LPS-induced IL-6, TNF-α, IL-1β, MCP-1, IFN-β and IL-10 production in Raw264.7 and mouse primary peritoneal macrophages.

Western Blot Analysis^[2]

Cell Line:

Raw264.7 cells
Concentration:

10 μM
Incubation Time:

0-180 min
Result:

Inhibited LPS-induced phosphorylation of IκBα (Ser32/36) in Raw264.7.

In Vivo

Micheliolide (10 or 20 mg/kg, i.p., a single dose for 2 h) attenuates the secretion of serum cytokines and protects against lung and liver tissue damage in LPS (10mg/kg, i.p., a single dose for 2 h)-challenged mice^[2].
Micheliolide (10 or 20 mg/kg, i.p., a single dose for 6 days) enhances the survival rate in mice following lethal septic shock^[2].
Micheliolide (30 mg/kg, i.p., every two days for 63 days) reduces swelling of the paws and suppresses the degeneration of articular cartilage in collagen-induced arthritis mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Acute peritonitis mouse model^[2]
Dosage:

10 or 20 mg/kg
Administration:

i.p., a single dose for 2 h
Result:

Suppressed LPS-induced production of proinflammatory cytokines, chemokines and IFN in macrophages and DCs, showing clearer alveolar wall and less inflammatory cell infiltration.

Animal Model:

Septic shock mouse model^[2]
Dosage:

10 or 20 mg/kg
Administration:

i.p., a single dose for 6 days
Result:

Protected mice against septic shock following lethal LPS challenge.

Animal Model:

Collagen-induced arthritis mice^[4]
Dosage:

30 mg/kg
Administration:

i.p., every two days for 63 days
Result:

Remited the pathological changes of collagen-induced arthritis and recovered the expression levels of M-CSF, TIMP-1 and C5/C5a in collagen-induced arthritis mice.

Chemical Information

CAS No. 68370-47-8
Appearance Solid
Molecular Weight 248.32
Formula C₁₅H₂₀O₃
Color White to off-white
SMILES

O=C(O[C@@]1([H])[C@@]2([H])CCC(C)=C3CC[C@@](C)(O)[C@@]31[H])C2=C
Structure Classification
- Terpenoids
- Other Monoterpenes
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

All WBRT-PCRHistological Imaging/StainingCell Proliferation/Viability AssayCell Migration/Invasion Assay

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Journal Impact Factor

J Transl Med

Micheliolide suppresses epithelial-mesenchymal transition of lens epithelial cells via downregulating matrix metalloproteinase 8 to ameliorate posterior capsular opacification. [Abstract]

2026 Feb 21;24(1):431. PMID: 41723479

Micheliolide purchased from MedChemExpress. Usage Cited in: J Transl Med. 2026 Feb 21;24(1):431. [Abstract]

Western blot and quantitative analysis of EMT-related markers in the capsular bags after ECLE combined with injection of saline or Micheliolide (MCL) (5 mM) into the anterior chamber.

Micheliolide purchased from MedChemExpress. Usage Cited in: J Transl Med. 2026 Feb 21;24(1):431. [Abstract]

qRT-PCR analysis of α-SMA, Col I, and FN in capsular bags after ECLE with or without anterior chamber injection of Micheliolide (MCL) (5 mM).

Micheliolide purchased from MedChemExpress. Usage Cited in: J Transl Med. 2026 Feb 21;24(1):431. [Abstract]

Representative HE staining shows cell proliferation and migration in the capsular bags following treatment with ECLE co-treated with an anterior chamber injection of saline or Micheliolide (MCL) (5 mM).

Micheliolide purchased from MedChemExpress. Usage Cited in: J Transl Med. 2026 Feb 21;24(1):431. [Abstract]

Viability test of LECs at gradient concentrations of Micheliolide (MCL) (1.25, 2.5, 5, 10, 20 μM).

Micheliolide purchased from MedChemExpress. Usage Cited in: J Transl Med. 2026 Feb 21;24(1):431. [Abstract]

Representative images of the scratch wound assay in LECs at 0, 24, and 48 hours. Cells were pretreated with or without 5 μmol/L Micheliolide (MCL) and then stimulated with 10 ng/mL TGF-β2.
PLoS Biol

A high-throughput behavioral screening platform for measuring chemotaxis by C. elegans. [Abstract]

2024 Jun 27;22(6):e3002672. PMID: 38935621
Pharmaceuticals (Basel)

Micheliolide Alleviates Hepatic Fibrosis by Inhibiting Autophagy in Hepatic Stellate Cells via the TrxR1/2-Mediated ROS/MEK/ERK Pathway. [Abstract]

2025 Feb 20;18(3):287. PMID: 40143066
Int Immunopharmacol

Micheliolide ameliorates severe acute pancreatitis in mice through potentiating Nrf2-mediated anti-inflammation and anti-oxidation effects. [Abstract]

2024 Oct 27;143(Pt 2):113490. PMID: 39467351
World Allergy Organ J

Protective effect of micheliolide against inflammation and oxidative stress in asthma through the MAPK/NF-κB signalling pathway. [Abstract]

2025 Jul 18;18(8):101091. PMID: 40718157
Carcinogenesis

EGFR-PKM2 signaling promotes the metastatic potential of nasopharyngeal carcinoma through induction of FOSL1 and ANTXR2. [Abstract]

2020 Jul 10;41(6):723-733. PMID: 31665243
J Biol Regul Homeost Agents

2023 Jul 20, 37(7): 3911-3919.
bioRxiv

An efficient behavioral screening platform classifies natural products and other chemical cues according to their chemosensory valence in C. elegans. [Abstract]

2024 Apr 3:2023.06.02.542933. PMID: 37333363

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (201.35 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.0271 mL

20.1353 mL

40.2706 mL

5 mM

0.8054 mL

4.0271 mL

8.0541 mL

10 mM

0.4027 mL

2.0135 mL

4.0271 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (10.07 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jia QQ, et al. Sesquiterpene lactones and their derivatives inhibit high glucose-induced NF-κB activation and MCP-1 and TGF-β1 expression in rat mesangial cells. Molecules. 2013 Oct 21;18(10):13061-77. [Content Brief]

[2]. Qin X, et al. Micheliolide inhibits LPS-induced inflammatory response and protects mice from LPS challenge. Sci Rep. 2016 Mar 17;6:23240. [Content Brief]

[3]. Viennois E, et al. Micheliolide, a new sesquiterpene lactone that inhibits intestinal inflammation and colitis-associated cancer. Lab Invest. 2014 Sep;94(9):950-65. [Content Brief]

[4]. Xu H, et al. Therapeutic effects of micheliolide on a murine model of rheumatoid arthritis. Mol Med Rep. 2015 Jan;11(1):489-93. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.0271 mL	20.1353 mL	40.2706 mL	100.6765 mL
	5 mM	0.8054 mL	4.0271 mL	8.0541 mL	20.1353 mL
	10 mM	0.4027 mL	2.0135 mL	4.0271 mL	10.0677 mL
	15 mM	0.2685 mL	1.3424 mL	2.6847 mL	6.7118 mL
	20 mM	0.2014 mL	1.0068 mL	2.0135 mL	5.0338 mL
	25 mM	0.1611 mL	0.8054 mL	1.6108 mL	4.0271 mL
	30 mM	0.1342 mL	0.6712 mL	1.3424 mL	3.3559 mL
	40 mM	0.1007 mL	0.5034 mL	1.0068 mL	2.5169 mL
	50 mM	0.0805 mL	0.4027 mL	0.8054 mL	2.0135 mL
	60 mM	0.0671 mL	0.3356 mL	0.6712 mL	1.6779 mL
	80 mM	0.0503 mL	0.2517 mL	0.5034 mL	1.2585 mL
	100 mM	0.0403 mL	0.2014 mL	0.4027 mL	1.0068 mL

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Micheliolide

Immunology/Inflammation Compound Library

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NF-κB Signaling Compound Library

PI3K/Akt/mTOR Compound Library

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TGF-beta/Smad Compound Library

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Natural Product and Natural Product-Like Compound Library

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3D Diverse Fragment Library

Publications Citing Use of MedChemExpress (MCE) Micheliolide

All NF-κB Isoforms

All PI3K Isoforms

All Akt Isoforms

All TGF-beta/Smad Isoforms

Biological Activity

Chemical Information