N-Acetyl-β-neuraminic acid methyl ester
Based on 1 Customer Validation
N-Acetyl-β-neuraminic acid methyl ester (N-Acetyl-Neuraminic acid (Sialic acid)) is an ester compound.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 22900-11-4
- Formula: C12H21NO9
- Molecular Weight:323.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
N-Acetyl-β-neuraminic acid methyl ester is a methyl ester derivative of N-acetyl-β-neuraminic acid, a member of the sialic acids family of nine-carbon monosaccharides[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 22900-11-4
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Appearance Solid
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Molecular Weight 323.30
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Formula C12H21NO9
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Color White to off-white
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SMILES
OC[C@@H](O)[C@H]([C@@]1([H])[C@@H]([C@H](C[C@](O)(O1)C(OC)=O)O)NC(C)=O)O
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Synonyms
N-Acetyl-Neuraminic acid (Sialic acid)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (309.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (15.47 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (15.47 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0931 mL | 15.4655 mL | 30.9310 mL | 77.3276 mL |
| 5 mM | 0.6186 mL | 3.0931 mL | 6.1862 mL | 15.4655 mL | |
| 10 mM | 0.3093 mL | 1.5466 mL | 3.0931 mL | 7.7328 mL | |
| 15 mM | 0.2062 mL | 1.0310 mL | 2.0621 mL | 5.1552 mL | |
| 20 mM | 0.1547 mL | 0.7733 mL | 1.5466 mL | 3.8664 mL | |
| 25 mM | 0.1237 mL | 0.6186 mL | 1.2372 mL | 3.0931 mL | |
| 30 mM | 0.1031 mL | 0.5155 mL | 1.0310 mL | 2.5776 mL | |
| 40 mM | 0.0773 mL | 0.3866 mL | 0.7733 mL | 1.9332 mL | |
| 50 mM | 0.0619 mL | 0.3093 mL | 0.6186 mL | 1.5466 mL | |
| 60 mM | 0.0516 mL | 0.2578 mL | 0.5155 mL | 1.2888 mL | |
| 80 mM | 0.0387 mL | 0.1933 mL | 0.3866 mL | 0.9666 mL | |
| 100 mM | 0.0309 mL | 0.1547 mL | 0.3093 mL | 0.7733 mL |
N-Acetyl-β-neuraminic acid methyl ester Related Classifications
HY-75279 Related Classifications
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