N-CBZ-Phe-Arg-AMC
Based on 1 Customer Validation
N-CBZ-Phe-Arg-AMC (Z-FR-AMC) is a cathepsin substrate used in assessment activity of lysosomal cathepsin enzymes.
For research use only. We do not sell to patients.
- Purity: 99.40%
- CAS No.: 65147-22-0
- Formula: C33H36N6O6
- Molecular Weight:612.68
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All Cathepsin Isoforms
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Biological Activity
N-CBZ-Phe-Arg-AMC (5 μM; 10-20 min) serves as a fluorescent substrate for trypsin, papain, and cathepsin B, enabling detection of apparent protease activity inhibition by myoglobin and hemin via reduced fluorescence signal[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 65147-22-0
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Appearance Solid
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Molecular Weight 612.68
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Formula C33H36N6O6
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Color White to off-white
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Synonyms
Z-FR-AMC
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Sequence
CBZ-Phe-Arg-AMC
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Sequence Shortening
CBZ-FR-AMC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (163.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Volle B, et al. Myoglobin inhibition of most protease activities measured with fluorescent substrates is an artifact!. Meat Sci. 1999;52(1):81-87. [Content Brief]
[2]. Xu P, et al. Ascorbic acid modulation of iron homeostasis and lysosomal function in trabecular meshwork cells. J Ocul Pharmacol Ther. 2014;30(2-3):246-253. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6322 mL | 8.1609 mL | 16.3217 mL | 40.8043 mL |
| 5 mM | 0.3264 mL | 1.6322 mL | 3.2643 mL | 8.1609 mL | |
| 10 mM | 0.1632 mL | 0.8161 mL | 1.6322 mL | 4.0804 mL | |
| 15 mM | 0.1088 mL | 0.5441 mL | 1.0881 mL | 2.7203 mL | |
| 20 mM | 0.0816 mL | 0.4080 mL | 0.8161 mL | 2.0402 mL | |
| 25 mM | 0.0653 mL | 0.3264 mL | 0.6529 mL | 1.6322 mL | |
| 30 mM | 0.0544 mL | 0.2720 mL | 0.5441 mL | 1.3601 mL | |
| 40 mM | 0.0408 mL | 0.2040 mL | 0.4080 mL | 1.0201 mL | |
| 50 mM | 0.0326 mL | 0.1632 mL | 0.3264 mL | 0.8161 mL | |
| 60 mM | 0.0272 mL | 0.1360 mL | 0.2720 mL | 0.6801 mL | |
| 80 mM | 0.0204 mL | 0.1020 mL | 0.2040 mL | 0.5101 mL | |
| 100 mM | 0.0163 mL | 0.0816 mL | 0.1632 mL | 0.4080 mL |
N-CBZ-Phe-Arg-AMC Related Classifications
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