Osteocalcin (human)
Based on 1 Customer Validation
Osteocalcin (Osteocalcin (1-49)) (human) is a vitamin K-dependent bone specific protein. Osteocalcin (human) is chemotactic for several of the cell types frequently found at bone remodeling surfaces.
For research use only. We do not sell to patients.
- Purity: 95.16%
- CAS No.: 136461-80-8
- Formula: C269H381N67O82S2
- Molecular Weight:5929.44
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Endogenous Metabolite Isoforms
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Biological Activity
Osteocalcin (1 nM-1 μM) is chemotactic for breast cancer cells and osteoblast-like osteogenic sarcoma cells as well as monocytes, and causes their unidirectional migration[2].
Osteocalcin peptide contains 49 amino acids, with subsequent breakdown to fragments 1-19, 20-49, 20-43, 1-43, and 44-49 in the liver, kidney, and serum[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 136461-80-8
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Appearance Solid
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Molecular Weight 5929.44
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Formula C269H381N67O82S2
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Color White to off-white
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Synonyms
Osteocalcin (1-49) (human)
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Sequence
Tyr-Leu-Tyr-Gln-Trp-Leu-Gly-Ala-Pro-Val-Pro-Tyr-Pro-Asp-Pro-Leu-{Gla}- Pro-Arg-Arg-{Gla}-Val-Cys-{Gla}-Leu-Asn-Pro-Asp-Cys-Asp-Glu-Leu-Ala-Asp-His-Ile-Gly-Phe-Gln-Glu-Ala-Tyr-Arg-Arg-Phe-Tyr-Gly-Pro-Val (Disulfide bridge:Cys23-Cys29)
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Sequence Shortening
YLYQWLGAPVPYPDPL-{Gla}-PRR-{Gla}-VC-{Gla}-LNPDCDELADHIGFQEAYRRFYGPV (Disulfide bridge:Cys23-Cys29)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (16.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (297 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Kuronen I, et al. Production of monoclonal and polyclonal antibodies against human osteocalcin sequences and development of a two-site ELISA for intact human osteocalcin. J Immunol Methods. 1993 Aug 9;163(2):233-40. [Content Brief]
[2]. Mundy GR, et al. Chemotactic activity of the gamma-carboxyglutamic acid containing protein in bone. Calcif Tissue Int. 1983;35(2):164-8. [Content Brief]
[3]. Colford JW, et al. Immunoradiometric assay for intact human osteocalcin(1-49) without cross-reactivity to breakdown products. Clin Chem. 1999 Apr;45(4):526-31. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.1686 mL | 0.8432 mL | 1.6865 mL | 4.2162 mL |
| 5 mM | 0.0337 mL | 0.1686 mL | 0.3373 mL | 0.8432 mL | |
| 10 mM | 0.0169 mL | 0.0843 mL | 0.1686 mL | 0.4216 mL | |
| 15 mM | 0.0112 mL | 0.0562 mL | 0.1124 mL | 0.2811 mL |
Osteocalcin (human) Related Classifications
HY-P2588 Related Classifications
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