p-SCN-Bn-HOPO
p-SCN-Bn-HOPO is a bifunctional chelator that forms stable octadentate complexes with Zr (IV) and 89Zr4+ via four 1,2-hydroxypyridone groups. p-SCN-Bn-HOPO conjugates with antibodies through the formation of thiourea bonds with lysine residues, and enables efficient 89Zr radiolabeling under mild conditions. p-SCN-Bn-HOPO inhibits bone uptake of free radiometals, stabilizes radiometal-antibody conjugates, and achieves PET imaging with low background and enhanced tumor-to-organ contrast. p-SCN-Bn-HOPO can be used in breast cancer-related research.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 1822331-55-4
- Formula: C43H45N9O12S
- Molecular Weight:911.94
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All Radionuclide-Drug Conjugates (RDCs) Isoforms
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Biological Activity
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RDC Bifunctional Chelator |
The conjugate of p-SCN-Bn-HOPO and Trastuzumab (HY-P9907) (administered for 7 days) retains approximately 90% stability in serum after 7 days, with a specific activity of up to approximately 2 mCi/mg[1].
89Zr-p-SCN-Bn-HOPO-trastuzumab (60 min; room temperature) retains high immunoreactivity against BT474 breast cancer cells, with an immunoreactivity score of 92.4%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:athymic nude mice (female)[2]
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Dosage:9.25-9.99 MBq (PET imaging); 0.59-0.67 MBq (biodistribution studies)
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Administration:i.v. tail vein injection
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Result:Showed good tumor uptake and markedly reduced bone uptake compared to the control conjugate.
Confirmed tumor uptake of 29.0 %ID/g at 24 h, 54.7 %ID/g at 72 h, 68.8 %ID/g at 120 h, 70.4 %ID/g at 168 h, 39.6 %ID/g at 216 h, and 61.9 %ID/g at 336 h.
Confirmed bone uptake of 2.6 %ID/g at 24 h, 2.7 %ID/g at 72 h, 2.0 %ID/g at 120 h, 2.5 %ID/g at 168 h, 2.5 %ID/g at 216 h, and 2.4 %ID/g at 336 h.
Achieved tumor:bone ratio of 25.8 at 336 h.
Achieved tumor:blood ratio of 14.4 at 336 h.
Chemical Information
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CAS No. 1822331-55-4
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Appearance Solid
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Molecular Weight 911.94
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Formula C43H45N9O12S
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Color White to off-white
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SMILES
O=C(N(CCC1=CC=C(N=C=S)C=C1)CCCN(C(C(N2O)=CC=CC2=O)=O)CCCCN(C(C(N3O)=CC=CC3=O)=O)CCCNC(C(N4O)=CC=CC4=O)=O)C(N5O)=CC=CC5=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (109.66 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.74 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (2.74 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.0966 mL | 5.4828 mL | 10.9656 mL | 27.4141 mL |
| 5 mM | 0.2193 mL | 1.0966 mL | 2.1931 mL | 5.4828 mL | |
| 10 mM | 0.1097 mL | 0.5483 mL | 1.0966 mL | 2.7414 mL | |
| 15 mM | 0.0731 mL | 0.3655 mL | 0.7310 mL | 1.8276 mL | |
| 20 mM | 0.0548 mL | 0.2741 mL | 0.5483 mL | 1.3707 mL | |
| 25 mM | 0.0439 mL | 0.2193 mL | 0.4386 mL | 1.0966 mL | |
| 30 mM | 0.0366 mL | 0.1828 mL | 0.3655 mL | 0.9138 mL | |
| 40 mM | 0.0274 mL | 0.1371 mL | 0.2741 mL | 0.6854 mL | |
| 50 mM | 0.0219 mL | 0.1097 mL | 0.2193 mL | 0.5483 mL | |
| 60 mM | 0.0183 mL | 0.0914 mL | 0.1828 mL | 0.4569 mL | |
| 80 mM | 0.0137 mL | 0.0685 mL | 0.1371 mL | 0.3427 mL | |
| 100 mM | 0.0110 mL | 0.0548 mL | 0.1097 mL | 0.2741 mL |
p-SCN-Bn-HOPO Related Classifications
HY-136952 Related Classifications
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