The trithorax-group gene in Drosophila little imaginal discs encodes a trimethylated histone H3 Lys4 demethylase

  • Nat Struct Mol Biol. 2007 Apr;14(4):344-6. doi: 10.1038/nsmb1217.
Joel C Eissenberg  1 ,  Min Gyu Lee ,  Jessica Schneider ,  Anne Ilvarsonn ,  Ramin Shiekhattar ,  Ali Shilatifard
Affiliations
  • 1. Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Blvd., St. Louis, Missouri 63104, USA.
PMID: 17351630 DOI: 10.1038/nsmb1217
Abstract

Histone H3 Lys4 (H3K4) is methylated by yeast Set1-COMPASS and its mammalian homolog, the MLL complex. Human JARID1d can demethylate trimethyl-H3K4 (H3K4me3). We identified Drosophila melanogaster little imaginal discs (Lid) as the JARID1d homolog. We report that Lid knockdown using RNA interference results in a specific genome-wide increase in H3K4me3 levels without affecting Other patterns of H3 methylation, and results in an altered distribution of the chromo-helicase protein Chd1.