Characterization of prednisone, prednisolone and their metabolites by gas chromatography-mass spectrometry

  • J Chromatogr. 1991 Apr 19;565(1-2):45-51. doi: 10.1016/0378-4347(91)80369-n.
G M Rodchenkov  1 ,  A N Vedenin ,  V P Uralets ,  V A Semenov
Affiliations
  • 1. Anti-Doping Centre, Central Institute of Sport, Moscow, U.S.S.R.
PMID: 1874892 DOI: 10.1016/0378-4347(91)80369-n
Abstract

Human urinary metabolites of the synthetic corticosteroids prednisone and prednisolone were detected in the course of gas chromatographic steroid profiling as methoxime-trimethylsilyl derivatives. Metabolites were provisionaly identified by combined gas chromatography-mass spectrometry. The major metabolites were 11-keto/11-hydroxy conversion products, 20-hydroxy and 4,5-dihydro analogues of the parent drugs. Cortisone, 6-hydroxy and fully saturated A-ring compounds were minor metabolites. Retention indices and mass spectral data are presented.

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