In vivo metabolism study of polygalic acid in rat using HPLC-ESI-MSn

  • Biomed Chromatogr. 2012 Feb;26(2):220-4. doi: 10.1002/bmc.1650.
Yun Ling  1 ,  Bin Wu ,  Ke Wang ,  Huihui Liu ,  Luying Chen ,  Mingsong Fan ,  Zhaolin Sun ,  Chenggang Huang
Affiliations
  • 1. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Zhangjiang, Pudong, Shanghai 201203, People's Republic of China.
PMID: 21618563 DOI: 10.1002/bmc.1650
Abstract

A very simple and direct method has been established for the determination of polygalic acid and its metabolites in rat urine based on HPLC coupled with electrospray ionization multi-stage tandem mass spectrometry (HPLC-ESI-MS(n)). The rats were administered a single dose (100 mg/kg) of polygalic acid by oral gavage. The urine samples were collected and purified through a C(18) solid-phase extraction cartridge, and then these pretreated samples were injected into a reversed-phase C(18) column with a gradient elution program, whereas acetonitrile-0.5% aqueous formic acid was used as mobile phase and detected by an on-line MS/MS system. As a result, the parent drug and its four metabolites were identified and characterized in rat urine for the first time by comparing their changes in molecular mass (ΔM), retention times and full-scan MS(n) spectra with those of the parent drug. A possible metabolic pathway of polygalic acid was investigated and proposed. More importantly, the results demonstrated that the newly developed method (HPLC-ESI-MS(n)) was sensitive, simple and suitable for the determination of polygalic acid and its metabolites in biological samples.

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