An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells

  • Cancer Lett. 2011 Dec 26;313(1):76-83. doi: 10.1016/j.canlet.2011.08.027.
Yun-Hee Kim  1 ,  Ho Jin Sung ,  Sukyoung Kim ,  Eun-Ok Kim ,  Ji Won Lee ,  Ju Young Moon ,  Kyungho Choi ,  Ji-Eun Jung ,  Yangsoon Lee ,  Sang Seok Koh ,  Sue Goo Rhee ,  Kyun Heo ,  In-Hoo Kim
Affiliations
  • 1. Molecular Imaging & Therapy Branch, Division of Convergence Technology, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 410-769, Republic of Korea.
PMID: 21963224 DOI: 10.1016/j.canlet.2011.08.027
Abstract

Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in Pancreatic Cancer and mediates the growth and metastasis of Pancreatic Cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K(D) of 77nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human Pancreatic Cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60% in an in vivo xenograft model with CFPAC-1 Pancreatic Cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human Pancreatic Cancer.

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