Assay Development for Protein Kinase Enzymes
J. Fraser Glickman
1
,
Sarine Markossian
1
,
Abigail Grossman
1
,
Hannah Baskir
1
,
Michelle Arkin
2
,
Douglas Auld
3
,
Christopher Austin
4
,
Jonathan Baell
5
,
Kyle Brimacombe
1
,
Thomas D.Y. Chung
6
,
Nathan P. Coussens
7
,
Jayme L. Dahlin
8
,
Viswanath Devanarayan
9
,
Timothy L. Foley
10
,
Marcie Glicksman
11
,
Kirill Gorshkov
12
,
Stefan Grotegut
13
,
Matthew D. Hall
1
,
Samuel Hoare
14
,
James Inglese
1
,
Philip W. Iversen
15
,
Madhu Lal-Nag
16
,
Zhuyin Li
12
,
Jason R. Manro
13
,
James McGee
17
,
Allison Norvil
13
,
Mackenzie Pearson
13
,
Terry Riss
18
,
Peter Saradjian
19
,
G. Sitta Sittampalam
20
,
Michael A. Tarselli
21
,
O. Joseph Trask Jr.
22
,
Jeffrey R. Weidner
23
,
Mary Jo Wildey
24
,
Kelli Wilson
1
,
Menghang Xia
1
,
Xin Xu
1
Affiliations
- 1. The Rockefeller University, New York, NY, 10065
PMID: 22553863
Abstract
Kinases are important drug targets that control cell growth, proliferation, differentiation and metabolism. This process involves phosphorylation of multiple substrates in cellular signal transduction pathways. Here, the authors provide a synopsis on assay technologies, mechanistic considerations for assay design and development pertaining to kinase Enzymes. This chapter, along with some of the earlier chapters in this manual on basics of enzyme assays, mechanism of action and purity and identity considerations, serves as an excellent resource for beginners in HTS applications.