Reverse-transcription PCR (RT-PCR)

  • Methods Enzymol. 2013;530:67-74. doi: 10.1016/B978-0-12-420037-1.00002-6.
Julia Bachman  1
Affiliations
  • 1. Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: [email protected].
PMID: 24034314 DOI: 10.1016/B978-0-12-420037-1.00002-6
Abstract

RT-PCR is commonly used to test for genetic diseases and to characterize gene expression in various tissue types, cell types, and over developmental time courses. This serves as a form of expression profiling, but typically as a candidate approach. RT-PCR is also commonly used to clone cDNAs for further use with other Molecular Biology techniques (e.g., see Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates and Circularized RT-PCR (cRT-PCR): analysis of RNA 5' ends, 3' ends, and poly(A) tails).

Keywords
Amplifying/cloning products; Polymerase chain reaction (PCR); Reverse transcriptase enzyme; Reverse-transcription PCR (RT-PCR); Unwinding RNA.