Immunostaining for rapid diagnosis of acute promyelocytic leukemia with the tetramethylrhodamine-5-isothiocyanate-conjugated anti-promyelocytic leukemia monoclonal antibody PG-M3

  • Arch Pathol Lab Med. 2013 Nov;137(11):1669-73. doi: 10.5858/arpa.2012-0565-OA.
Khaled M Alayed  1 ,  L Jeffrey Medeiros ,  Danh Phan ,  Chinemerem Ojiaku ,  Jyoti Patel ,  Jan Paul V Yap ,  Yen McCord ,  Janaya S Woods ,  Sergej Konoplev ,  Carlos E Bueso-Ramos ,  Steven R Reyes
Affiliations
  • 1. From the Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston (Drs Alayed, Medeiros, Konoplev, and Bueso-Ramos, Mr Phan, Ms Ojiaku, Ms Patel, Ms McCord, Mr Yap, and Ms Woods); and the Department of Pathology, College of Medicine, King Saud University, Riyadh, Saudi Arabia (Dr Alayed).
PMID: 24168508 DOI: 10.5858/arpa.2012-0565-OA
Abstract

Context: Anti-promyelocytic leukemia (PML) immunofluorescence staining is a known diagnostic tool for rapid diagnosis of acute promyelocytic leukemia (APL).

Objective: We describe our methods using the recently developed, commercially available, tetramethylrhodamine-5-isothiocyanate-labeled PG-M3 anti-PML antibody for APL testing.

Design: Immunofluorescence staining with the tetramethylrhodamine-5-isothiocyanate-labeled PG-M3 antibody was used to detect PML-RARA in bone marrow aspirate and/or peripheral blood smears from 30 patients with acute leukemia. The results were compared with those of concurrent testing with our in-house polyclonal anti-PML antibody and with established tests.

Results: All APL cases showed a positive (fine/microgranular) immunofluorescence staining pattern, whereas non-APL cases showed a negative (chunky/macrogranular) pattern. These results, which were available within 2 hours, were validated by testing with the polyclonal anti-PML antibody and with established cytogenetic and molecular testing methods.

Conclusions: We validated the utility of the tetramethylrhodamine-5-isothiocyanate-labeled anti-PML antibody PG-M3 for the diagnosis of APL. Our results indicate that immunofluorescence staining with this antibody is a rapid and reliable method for the diagnosis of APL.

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