C- and O-glycosyl flavonoids in Sanguinello and Tarocco blood orange (Citrus sinensis (L.) Osbeck) juice: Identification and influence on antioxidant properties and acetylcholinesterase activity

  • Food Chem. 2016 Apr 1:196:619-27. doi: 10.1016/j.foodchem.2015.09.098.
Davide Barreca  1 ,  Giuseppe Gattuso  2 ,  Giuseppina Laganà  1 ,  Ugo Leuzzi  1 ,  Ersilia Bellocco  1
Affiliations
  • 1. Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
  • 2. Dipartimento di Scienze Chimiche, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. Electronic address: [email protected].
PMID: 26593535 DOI: 10.1016/j.foodchem.2015.09.098
Abstract

Sanguinello and Tarocco are the blood orange (Citrus sinensis (L.) Osbeck) cultivars most diffused worldwide. Reversed phase liquid chromatography coupled with MS-MS analysis showed that these two varieties have a similar chromatographic pattern, characterised by the presence of C- and O-glycosyl Flavonoids. Of the two, Sanguinello was found to be far richer in Flavonoids than Tarocco. In the juices, twelve individual components were identified for the first time, namely, four C-glycosyl Flavones (lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin), three flavonol derivatives (quercetin-3-O-(2-rhamnosyl)-rutinoside, quercetin-3-O-hexoside, quercetin 3-hydroxy-3-methylglutaryl-glycoside), an O-triglycosyl flavanone (narirutin 4'-O-glucoside) and a flavone O-glycosides (chrysoeriol 7-O-neoesperidoside). Moreover, the influence of the identified C- and O-glycosyl Flavonoids on the antioxidant and acetylcholinesterase activity of these juices has been evaluated.

Keywords
Acetylcholinesterase activity; Antioxidant activity; Citrus sinensis (L.) Osbeck; Flavonoids; HPLC–DAD–ESI-MS-MS; Sanguinello and Tarocco blood orange.
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