Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green

  • Sci Rep. 2016 Jun 9:6:27525. doi: 10.1038/srep27525.
Yoshiaki Shinden  1  2 ,  Hiroki Ueo  1  3 ,  Taro Tobo  4 ,  Ayako Gamachi  5 ,  Mitsuaki Utou  4 ,  Hisateru Komatsu  1 ,  Sho Nambara  1 ,  Tomoko Saito  1 ,  Masami Ueda  1 ,  Hidenari Hirata  1 ,  Shotaro Sakimura  1 ,  Yuki Takano  1 ,  Ryutaro Uchi  1 ,  Junji Kurashige  1 ,  Sayuri Akiyoshi  1 ,  Tomohiro Iguchi  1 ,  Hidetoshi Eguchi  1 ,  Keishi Sugimachi  1 ,  Yoko Kubota  6 ,  Yuichiro Kai  6 ,  Kenji Shibuta  6 ,  Yuko Kijima  2 ,  Heiji Yoshinaka  2 ,  Shoji Natsugoe  2 ,  Masaki Mori  7 ,  Yoshihiko Maehara  3 ,  Masayo Sakabe  8 ,  Mako Kamiya  8 ,  John W Kakareka  9 ,  Thomas J Pohida  9 ,  Peter L Choyke  10 ,  Hisataka Kobayashi  10 ,  Hiroaki Ueo  6 ,  Yasuteru Urano  8  11 ,  Koshi Mimori  1
Affiliations
  • 1. Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
  • 2. Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
  • 3. Department of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
  • 4. Department of Pathology, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan.
  • 5. Department of Pathology, Oita University, Yufu 879-5593, Japan.
  • 6. Ueo Breast Surgery Hospital, Oita 870-0854, Japan.
  • 7. Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita 565-0871, Japan.
  • 8. Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • 9. Signal Processing and Instrumentation Section, Division of Computational Bioscience, Center of Information Technology, National Institutes of Health, Bethesda, MD 20892-5624, USA.
  • 10. Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1088, USA.
  • 11. CREST, AMED, Tokyo 100-0004, Japan.
PMID: 27277343 DOI: 10.1038/srep27525
Abstract

Sentinel lymph node biopsy is performed as a standard procedure in Breast Cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic Lymph Nodes in Breast Cancer. One hundred and forty-nine Lymph Nodes from 38 Breast Cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing Breast Cancer cells in Lymph Nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of Cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.