Homoisoflavonoids and the Antioxidant Activity of Ophiopogon japonicus Root

  • Iran J Pharm Res. 2017 Winter;16(1):357-365.
Yancui Wang  1 ,  Feng Liu  2  3 ,  Zongsuo Liang  1 ,  Liang Peng  1 ,  Bangqing Wang  1 ,  Jing Yu  1 ,  Yingying Su  4 ,  Cunde Ma  4
Affiliations
  • 1. College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China.
  • 2. School of Pharmacy, Health Science Center, Xi›an Jiaotong University, Xi'an, Shaanxi Province, China.
  • 3. Shaanxi institute of international trade & commerce, Xiangyang 712046, China.
  • 4. Shaanxi Buchang Pharmaceutical Co, Ltd, Shaanxi, China.
PMID: 28496489
Abstract

The root of Ophiopogon japonicus has been used as a traditional Chinese medicine and also a functional food ingredient for a long time in China. In the present study, 17 different homoisoflavonoid compounds were identified in the root extract of O. japonicus by HPLC-DAD and LCMS/MS analyses. The antioxidant activity of the of chloroform/methanol (1:1, v/v), methanol and 70% ethanol extracts, and two major isolated homoisoflavonoid compounds (methylophiopogonanone A and methylophiopogonanone B) from O. japonicus root were investigated by various in-vitro assays. Methylophiopogonanone B showed the highest antioxidant ability according to four antioxidant methods. Among the extracts, the chloroform/methanol extract which contained high amounts of homoisoflavonoids was found to exhibit the strongest antioxidant activity. The results showed that O. japonicus root can be regarded as a potential source of homoisoflavonoids and natural antioxidant.

Keywords
Antioxidant activity; Homoisoflavonoids; LCMS/MS analysis; Ophiopogon japonicas.
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