Chemical Constituents with Proprotein Convertase Subtilisin/Kexin Type 9 mRNA Expression Inhibitory Activity from Dried Immature Morus alba Fruits

  • J Agric Food Chem. 2017 Jul 5;65(26):5316-5321. doi: 10.1021/acs.jafc.7b02088.
Pisey Pel  1 ,  Hee-Sung Chae  1 ,  Piseth Nhoek  1 ,  Young-Mi Kim  1 ,  Young-Won Chin  1
Affiliations
  • 1. College of Pharmacy and Integrated Research Institute for Drug Development, Dongguk University-Seoul , 32, Dongguk-lo, Goyang-si, Gyeonggi-do 10326, Korea.
PMID: 28649844 DOI: 10.1021/acs.jafc.7b02088
Abstract

Phytochemical investigation for a chloroform-soluble extract of dried Morus alba fruits, selected by proprotein convertase subtilisin-kexin type 9 (PCSK9) mRNA expression monitoring assay in HepG2 cells, led to the isolation of a new benzofuran, isomoracin D (1), and a naturally occurring compound, N-(N-benzoyl-l-phenylalanyl)-l-phenylalanol (2), along with 13 known compounds (3-15). All of the structures were established by NMR spectroscopic data as well as MS analysis. Of the isolates, moracin C (7) was found to inhibit PCSK9 mRNA expression with an IC50 value of 16.8 μM in the HepG2 cells.

Keywords
Morus alba fruits; N-(N-phenzoyl-l-phenylalanyl)-l-phenylalanol; PCSK9 mRNA expression; isomoracin D; moracin C.
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