Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA

  • Chem Commun (Camb). 2017 Aug 3;53(63):8910-8913. doi: 10.1039/c7cc05159j.
Pawan Kumar  1 ,  Afaf H El-Sagheer  2 ,  Lynda Truong  1 ,  Tom Brown  1
Affiliations
  • 1. Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK. [email protected].
  • 2. Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK. [email protected] and Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt.
PMID: 28748236 DOI: 10.1039/c7cc05159j
Abstract

Oligonucleotides containing internal triazole-3'-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified oligonucleotides, and significantly better than oligonucleotides containing triazole alone. In contrast LNA on the 5'-side of the triazole does not stabilise duplexes. Triazole-LNA confers great resistance towards enzymatic degradation relative to LNA alone.

Products