Separation of six compounds including two n-butyrophenone isomers and two stibene isomers from Rheum tanguticum Maxim by recycling high speed counter-current chromatography and preparative high-performance liquid chromatography

  • J Sep Sci. 2018 Oct;41(19):3660-3668. doi: 10.1002/jssc.201800411.
Tao Chen  1 ,  Xue Yang  1  2 ,  Nana Wang  1  2 ,  Hongmei Li  1 ,  Jingyang Zhao  1 ,  Yulin Li  1
Affiliations
  • 1. Qinghai Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xining, P. R. China.
  • 2. Savaid Medical School, University of the Chinese Academy of Sciences, Beijing, P. R. China.
PMID: 30058764 DOI: 10.1002/jssc.201800411
Abstract

Six compounds including two n-butyrophenone isomers and two stibene isomers were obtained from Rheum tanguticum Maxim. Two n-butyrophenone isomers with a separation factor of 1.14 were successfully separated by recycling high-speed counter-current chromatography after ten cycles. Two stibene isomers were successfully separated by preparative high-performance liquid chromatography. High-performance liquid chromatography analysis showed that the purities of the compounds were all over 98%. These compounds were identified as lindleyin, isolindleyin, resveratrol-4'-O-(2″-O-galloyl)-glucopyranoside, resveratrol-4'-O-(6''-O-galloyl)-glucopyranoside, emodin 1-O-β-d-glucoside, and 3,5-dihydroxy-4'-methoxystilbene-3-O-β-d-glucopyranoside. The results indicated that recycling high-speed counter-current chromatography and preparative high-performance liquid chromatography could be effective combination for the preparation of bioactive compounds from Rheum tanguticum Maxim.

Keywords
Rheum tanguticum Maxim; high-speed counter-current chromatography; n-butyrophenone isomers; preparative high-performance liquid chromatography; stibene isomers.
Products