2'- O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA

  • Molecules. 2018 Oct 9;23(10):2572. doi: 10.3390/molecules23102572.
Thananjeyan Balasubramaniyam  1 ,  Takumi Ishizuka  2 ,  Chao-Da Xiao  3  4 ,  Hong-Liang Bao  5 ,  Yan Xu  6
Affiliations
  • 1. Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. [email protected].
  • 2. Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. [email protected].
  • 3. Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. [email protected].
  • 4. The key Laboratory of Optimal Utilization of Natural Medicine Resources, School of Pharmaceutical Sciences, Guizhou Medical University, University Town, Guian New District, Guiyang 550025, China. [email protected].
  • 5. Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. [email protected].
  • 6. Division of Chemistry, Department of Medical Sciences, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan. [email protected].
PMID: 30304782 DOI: 10.3390/molecules23102572
Abstract

In contrast to Z-DNA that was stabilized and well-studied for its structure by chemical approaches, the stabilization and structural study of Z-RNA remains a challenge. In this study, we developed a Z-form RNA stabilizer m⁸Gm, and demonstrated that incorporation of m⁸Gm into RNA can markedly stabilize the Z-RNA at low salt conditions. Using the m⁸Gm-contained Z-RNA, we determined the structure of Z-RNA and investigated the interaction of protein and Z-RNA.

Keywords
NMR; Z-RNA structure; circular dichroism; synthesis of oligonucleotide.
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