Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

  • J Vis Exp. 2019 Oct 26:(152). doi: 10.3791/60168.
Shahin Shabanipour  1 ,  Azadeh Dalvand  1 ,  Xiaodan Jiao  1 ,  Maryam Rahimi Balaei  1 ,  Seung H Chung  2 ,  Jiming Kong  3 ,  Marc R Del Bigio  4 ,  Hassan Marzban  5
Affiliations
  • 1. Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • 2. Department of Oral Biology, University of Illinois at Chicago.
  • 3. Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • 4. The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
  • 5. Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; [email protected].
PMID: 31710031 DOI: 10.3791/60168
Abstract

The use of primary cell cultures has become one of the major tools to study the nervous system in vitro. The ultimate goal of using this simplified model system is to provide a controlled microenvironment and maintain the high survival rate and the natural features of dissociated neuronal and nonneuronal cells as much as possible under in vitro conditions. In this article, we demonstrate a method of isolating primary neurons from the developing mouse cerebellum, placing them in an in vitro environment, establishing their growth, and monitoring their viability and differentiation for several weeks. This method is applicable to embryonic neurons dissociated from cerebellum between embryonic days 12-18.