Citrinin against breast cancer: A cytogenotoxicological study

  • Phytother Res. 2021 Jan;35(1):504-516. doi: 10.1002/ptr.6830.
José Williams Gomes de Oliveira Filho  1  2  3 ,  Teresinha de Jesus Aguiar Dos Santos Andrade  4 ,  Rosália Maria Tôrres de Lima  1 ,  Antonielly Campinho Dos Reis  2 ,  Dulce Helena Siqueira Silva  5 ,  José Victor de Oliveira Santos  2 ,  Ag-Anne Pereira Melo de Menezes  2 ,  Ana Maria Oliveira da Mata  2 ,  Ana Carolina Soares Dias  6 ,  Marcus Vinícius Oliveira Barros de Alencar  2 ,  Márcia Fernanda Correia Jardim Paz  2 ,  Lina Clara Gayoso E Almendra Ibiapina Moreno  7 ,  Muhammad Torequl Islam  8  9 ,  Mohammad S Mubarak  10 ,  João Marcelo de Castro E Sousa  1  7 ,  Ana Amélia de Carvalho Melo Cavalcante  1  7
Affiliations
  • 1. Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí - UFPI, Teresina, Piauí, Brazil.
  • 2. Laboratory of Research in Toxicological Genetics - LAPGENIC, Federal University of Piauí, Teresina, Piauí, Brazil.
  • 3. Federal Institute of Piauí (IFPI), Teresina, Piauí, Brazil.
  • 4. Nucleus of Applied Research to Sciences (NIAC), Federal Institute of Education, Science and Technology of Maranhão (IFMA), Timon, Maranhão, Brazil.
  • 5. Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Products (NuBBE), Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
  • 6. Laboratory of Genetics and Molecular Biology, Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • 7. Laboratory of Pharmaceutical Nanosystems - NANOSFAR, Federal University of Piauí, Teresina, Piauí, Brazil.
  • 8. Laboratory of Theoretical and Computational Biophysics, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
  • 9. Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
  • 10. Department of Chemistry, The University of Jordan, Amman, Jordan.
PMID: 32869401 DOI: 10.1002/ptr.6830
Abstract

Breast Cancer is one of the most lethal types of Cancer and a leading cause of mortality among Women worldwide. Citrinin (CIT), a polyketide extracted from the fungus Penicillium citrinum, exhibits a wide range of biological activities such as Antibacterial, Antifungal, and cytotoxic effects. The aim of the current study was to evaluate the antitumoral effects of CIT against 7,12-dimethylbenzanthracene (DMBA)-induced mammary carcinoma in Swiss mice For this, CIT, DMBA and the standard cyclophosphamide (CPA) induced behavioral changes in experimental Animals, and these changes were screened by using the rota rod and open field tests. Additionally, hematological, biochemical, immuno-histochemical, and histopathological analyses were carried out. Results suggest that CIT did not alter behavioral, hematological, and biochemical parameters in mice. DMBA induced invasive mammary carcinoma and showed genotoxic effects in the breasts, bone marrow, lymphocytes, and hepatic cells. It also caused mutagenic effects in the formation of micronuclei, bridges, shoots, and binucleate cells in bone marrow and liver. CIT and CPA genotoxic effects were observed after 3 weeks of therapy, where CIT exhibited a repair capacity and induced significant apoptotic damage in mouse lymphocytes. In conclusion, CIT showed antitumoral effects in Swiss mice, possibly through induction of Apoptosis.

Keywords
Mus musculus; anticancer drug; breast cancer; citrinin; mycotoxin.
Products