Preparation, characterization, and bioactivity evaluation of oligosaccharides from Atractylodes lancea (Thunb.) DC

  • Carbohydr Polym. 2022 Feb 1;277:118854. doi: 10.1016/j.carbpol.2021.118854.
Ying-Yue Zhang  1 ,  Dan Zhuang  2 ,  Hui-Yang Wang  2 ,  Chun-Yao Liu  2 ,  Guang-Ping Lv  3 ,  Li-Juan Meng  4
Affiliations
  • 1. School of Life Science, Nanjing Normal University, Nanjing 210023, PR China.
  • 2. School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, PR China.
  • 3. School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210023, PR China; National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, PR China. Electronic address: [email protected].
  • 4. Department of Geriatric Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China. Electronic address: [email protected].
PMID: 34893263 DOI: 10.1016/j.carbpol.2021.118854
Abstract

Sixteen oligosaccharide monomers with the degree of polymerization 3 to 18 (DP 3 to DP 18) and three active fractions (DP 3-9, DP 8-11, and DP 11-17) were separated from Atractylodes lancea (Thunb.) DC. by optimized fast protein liquid chromatography coupled with refractive index detector (FPLC-RID) and preparation hydrophilic interaction chromatography (Pre-HILIC). Gas chromatography-mass spectrometer (GC-MS), liquid chromatography tandem mass spectrometry (LC-MS/MS), nuclear magnetic resonance (NMR) spectroscopy, and methylation analysis showed that the oligosaccharide in A. lancea was 1-kestose [β-D-fructofuranosyl-(2 → 1)-β-D-fructofuranosyl-(2 → 1)-α-D-glucopyranoside] (inulin-type fructooligosaccharides, FOS). Particularly, DP 3-9 showed the best capacity in stimulating phagocytic, NO, and cytokines production on RAW264.7 cells than any Other purified oligosaccharide monomers and active fractions. It could also activate T-cells in Peyer's patch cells and enhance the production of colony stimulation factors. Besides, FPLC-RID showed a good capacity for large-scale preparation of DP 3-9 with the recovery of more than 93%. The bioactivity of sixteen FOS monomers (DP 3 to DP 18) and three FOS fractions (DP 3-9, DP 8-11, and DP 11-17) investigated in this study are beneficial for the utilization of FOS as a functional ingredient in novel product development.

Keywords
Atractylodes lancea (Thunb.) DC.; Fructooligosaccharides; Immunoregulatory activities; Intestinal immune system modulating activity; Rapid enrichment method.
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