A protocol to characterize zebrafish LGP2 as a dual regulator of IFN response during viral infection

  • STAR Protoc. 2022 Dec 16;3(4):101844. doi: 10.1016/j.xpro.2022.101844.
Xiu-Ying Gong  1 ,  Yi-Bing Zhang  2
Affiliations
  • 1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 10049, China. Electronic address: [email protected].
  • 2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 10049, China; The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China; Key Laboratory of Aquaculture Disease Control of Ministry of Agriculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: [email protected].
PMID: 36595883 DOI: 10.1016/j.xpro.2022.101844
Abstract

Here, we present a protocol to characterize zebrafish LGP2 as a dual regulator of interferon (IFN) response. We detail in vivo assays using time-lapse comparison of IFN response between wild-type and lgp2 knockout zebrafish following spring viraemia of carp virus (SVCV) Infection. We also describe in vitro assays including titration of Infection duration in SVCV-infected fish cells to determine changes in IFN response. This protocol is effective to illuminate a regulatory switch of LGP2 in fish cells toward virus Infection. For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1.

Keywords
Immunology; Model Organisms; Molecular Biology; Protein Biochemistry; Signal Transduction.
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