Effects of a hemoregulatory peptide (HP5b) on erythroid and myelopoietic colony formation in vitro

  • Scand J Haematol. 1986 Aug;37(2):79-86. doi: 10.1111/j.1600-0609.1986.tb01777.x.
L Kreja ,  P Hågå ,  N Muller-Bérat ,  O D Laerum ,  O Sletvold ,  W R Paukovits
PMID: 3764340 DOI: 10.1111/j.1600-0609.1986.tb01777.x
Abstract

A hemoregulatory peptide (HP5b) associated with mature human granulocytes has been investigated for inhibitory effects on murine hemopoietic stem cells in vitro. Both highly purified peptide from natural sources and a synthetic analog peptide have been investigated in parallel. Strong inhibitory effects were found on myelopoietic colony formation in the dose range 10(-13)-10(-5) mol/l. On exceeding this dose, the inhibitory effect disappeared. Moderate to slight inhibitory effects on erythroid colony formation (BFU-E and CFU-E) from adult Animals were only seen in 1,000 X the optimal doses for myelopoiesis. No effect was found on CFU-E from fetal liver. The peptide thus has a selective effect on myelopoiesis in a certain dose range. A regulatory mechanism for the peptide on hemopoiesis is proposed.

Products