Chemical Analysis and Antidiabetic Potential of a Decoction from Stevia serrata Roots

  • J Nat Prod. 2023 Sep 22. doi: 10.1021/acs.jnatprod.3c00711.
Sofía Padilla-Mayne  1 ,  Berenice Ovalle-Magallanes  1 ,  Mario Figueroa  1 ,  Edelmira Linares  2 ,  Robert Bye  2 ,  Isabel Rivero-Cruz  1 ,  Martín González-Andrade  3 ,  Rodrigo Aguayo-Ortiz  1 ,  Rachel Mata  1
Affiliations
  • 1. Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México 04510, México.
  • 2. Jardín Botánico, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México 04510, México.
  • 3. Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de México 04510, México.
PMID: 37738100 DOI: 10.1021/acs.jnatprod.3c00711
Abstract

A decoction of the roots (31.6-316 mg/kg) from Stevia serrata Cav. (Asteraceae) as well as the main component (5-150 mg/kg) showed hypoglycemic and antihyperglycemic effects in mice. The fractionation of the active extract led to the isolation of dammaradiene acetate (1), stevisalioside A (2), and three new chemical entities characterized by spectroscopic methods and named stevisaliosides B-D (3-5). Glycoside 2 (5 and 50 mg/kg) decreased blood glucose levels and the postprandial peak during oral glucose and Insulin tolerance tests in STZ-hyperglycemic mice. Compounds 1-5 were tested also against PTP1B1-400 and showed IC50 values of 1180.9 ± 0.33, 526.8 ± 0.02, 532.1 ± 0.03, 928.2 ± 0.39, and 31.8 ± 1.09 μM, respectively. Compound 5 showed an IC50 value comparable to that of ursolic acid (IC50 = 30.7 ± 0.00 μM). Docking studies revealed that 2-5 and their aglycones bind to PTP1B1-400 in a pocket formed by the C-terminal region. The volatilome of S. serrata was characterized by a high content of (E)-longipinene, spathulenol, guaiadiene, seychellene, and aromandendrene. Finally, a UHPLC-UV method was developed and validated to quantify the content of 2 in the decoction of the plant.

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