Nitrogenous compounds from Aesculus wilsonii seeds

  • Fitoterapia. 2024 Jan:172:105783. doi: 10.1016/j.fitote.2023.105783.
Huina Cao  1 ,  Jingya Ruan  2 ,  Xiaoyan Cao  1 ,  Yaqi Zhang  1 ,  Jia Hao  1 ,  Yuzheng Wu  1 ,  Yi Zhang  3 ,  Tao Wang  4
Affiliations
  • 1. Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
  • 2. Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China.
  • 3. Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China. Electronic address: [email protected].
  • 4. Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Component-Based Chinese Medicine, Tianjin 301617, China. Electronic address: [email protected].
PMID: 38110127 DOI: 10.1016/j.fitote.2023.105783
Abstract

Eight nitrogenous compounds including five undescribed ones, aeswilnitrousol A (1), aeswilnitrousosides BD (2-4), and 6-(2-hydroxy-3-methylbutylamino)-8-oxoadenine (5) were isolated from the seeds of Aesculus wilsonii. Their structures and absolute configurations were established based on spectroscopic determination, calculated electronic circular dichroism (ECD) analysis, as well as chemical reaction methods. Among the three known compounds, 7 and 8 were obtained from the Aesculus genus for the first time, and 6 was gained from this plant initially. The 13C NMR data of 7 and 8 were reported for the first time. Moreover, the inhibitory effect of all the isolates against LPS-induced nitric oxide production in RAW264.7 macrophages was evaluated. As a result, compounds 2 and 8 exhibited anti-inflammatory activity in a concentration-dependent manner at 10, 25, and 50 μM.

Keywords
NO production inhibition; Nitrogenous compounds; The seeds of Aesculus wilsonii Rehd.
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