Macrophage metabolic reprogramming ameliorates diabetes-induced microvascular dysfunction

  • Redox Biol. 2024 Nov 29:79:103449. doi: 10.1016/j.redox.2024.103449.
Qiu-Yang Zhang  1 ,  Hui-Ying Zhang  2 ,  Si-Guo Feng  2 ,  Mu-Di Yao  3 ,  Jing-Juan Ding  2 ,  Xiu-Miao Li  4 ,  Rong Ye  2 ,  Qing Liu  2 ,  Jin Yao  5 ,  Biao Yan  6
Affiliations
  • 1. The Affiliated Eye Hospital, Nanjing Medical University, Nanjing, 210000, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, 210000, China.
  • 2. The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, 210000, China.
  • 3. Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.
  • 4. The Affiliated Eye Hospital, Nanjing Medical University, Nanjing, 210000, China.
  • 5. The Affiliated Eye Hospital, Nanjing Medical University, Nanjing, 210000, China; The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, 210000, China. Electronic address: [email protected].
  • 6. Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China; Eye Institute and Department of Ophthalmology, Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, 200030, China. Electronic address: [email protected].
PMID: 39647239 DOI: 10.1016/j.redox.2024.103449
Abstract

Macrophages play an important role in the development of vascular diseases, with their homeostasis closely linked to metabolic reprogramming. This study aims to explore the role of circular RNA-mediated epigenetic remodeling in maintaining macrophage homeostasis during diabetes-induced microvascular dysfunction. We identified a circular RNA, circRNA-sperm antigen with calponin homology and coiled-coil domains 1 (cSPECC1), which is significantly up-regulated in diabetic retinas and in macrophages under diabetic stress. cSPECC1 knockdown in macrophages attenuates M1 macrophage polarization and disrupts macrophage-endothelial crosstalk in vitro. cSPECC1 knockdown in macrophages mitigates diabetes-induced retinal inflammation and ameliorates retinal vascular dysfunction. Mechanistically, cSPECC1 regulates GPX2 expression by recruiting eIF4A3, enhancing GPX2 mRNA stability and altering arachidonic acid metabolism. The metabolic intermediate 12-HETE has emerged as a key mediator, regulating both macrophage homeostasis and the crosstalk between macrophages and endothelial cells. Exogenous 12-HETE supplementation interrupts the anti-angiogenic effects of cSPECC1 knockdown. Collectively, circSPECC1 emerges as a novel regulator of macrophage-mediated vascular integrity and inflammation. Targeting the metabolic reprogramming of macrophages presents a promising therapeutic strategy for mitigating diabetes-induced vascular dysfunction.

Keywords
Circular RNAs; Diabetic retinopathy; Macrophage homeostasis; Metabolic reprogramming; Vascular dysfunction.
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