A TPP-OH-based near-infrared fluorescent nanoprobe for cellular and in vivo H2S detection and imaging

  • Colloids Surf B Biointerfaces. 2025 Oct:254:114848. doi: 10.1016/j.colsurfb.2025.114848.
Shanshan Fan  1 ,  Shengsheng Cui  1 ,  Cheng Cao  1 ,  Xinni Pan  1 ,  Shujing Lin  1 ,  Yuanli Wang  2 ,  Yongguang Lu  3 ,  Yanlei Liu  4
Affiliations
  • 1. Institute of Intelligent Health Diagnosis and Treatment, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
  • 2. The First People's Hospital of Qinzhou, The Tenth Affiliated Hospital of Guangxi Medical University, Qinzhou 535000, China.
  • 3. The First People's Hospital of Qinzhou, The Tenth Affiliated Hospital of Guangxi Medical University, Qinzhou 535000, China. Electronic address: [email protected].
  • 4. Institute of Intelligent Health Diagnosis and Treatment, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China. Electronic address: [email protected].
PMID: 40513213 DOI: 10.1016/j.colsurfb.2025.114848
Abstract

The physiological and pathological processes of many diseases are influenced by hydrogen sulfide (H2S), a physiologically active gaseous signaling molecule. Near-infrared fluorescent (NIR) probes are valued for their high specificity, sensitivity, simplicity, and safety. A near-infrared fluorescent probe (TPNPs), sensitive to H2S, was designed and synthesized from 4-(10,15,20-triphenylporphyrin-5-yl)phenol (TPP-OH). Due to the creation of hydroxyl groups from ether thiolation, it was discovered that Na2S treatment of TPNPs with Na2S as the H2S donor produced a large "turn-on" near-infrared fluorescence response. Additionally, the fluorescence intensity of TPNPs at 650 nm was significantly enhanced with the increase of Na2S content. The fluorescence intensity of TPNPs at 650 nm showed a strong linear relationship with Na2S concentration within the concentration range of 0-50 μM, with a detection limit of 0.88 μM. Furthermore, TPNPs were effectively used to investigate exogenous and endogenous H2S in mice and live cells.

Keywords
Fluorescence imaging; Hydrogen sulfide; Near-infrared fluorescent probes; Selectivity; “Turn-on” response.
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