Targeted NanoBiT Screening Identifies a Novel Interaction Between SNAPIN and Influenza A Virus M1 Protein

  • Biology (Basel). 2025 Dec 11;14(12):1770. doi: 10.3390/biology14121770.
Xiaoxuan Zhang  1  2  3 ,  Huanhuan Wang  1  2  3 ,  Conghui Zhao  1  2  3 ,  Wenjun Shi  4 ,  Faxin Wen  1  2  3 ,  Haoxi Qiang  1  2  3 ,  Sha Liu  1  2  3 ,  Peilin Li  1  2  3 ,  Xinhui Chen  1  2  3 ,  Chunping Zhang  1  2  3 ,  Jiacheng Huang  1  2  3 ,  Yang Wang  1  2  3 ,  Ziyi Zhang  1  2  3 ,  Shujie Ma  1  2  3
Affiliations
  • 1. Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • 2. Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • 3. Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • 4. Technology Center of Qingdao Customs, Qingdao 266000, China.
PMID: 41463543 DOI: 10.3390/biology14121770
Abstract

Influenza A virus (IAV) poses a significant threat due to its rapid evolution through gene mutations and reassortments. Understanding host-virus protein interactions is vital for developing countermeasures. In this study, we developed a live-cell screening platform using the NanoBiT system for rapid discovery of host-virus protein-protein interactions (PPIs). Novel interactions between the host factor SNAPIN and the viral M1, M2 and NS2 were identified using this system. We confirmed the platform's reliability by validating the SNAPIN-M1 interaction using independent methods including co-immunoprecipitation (Co-IP) and Glutathione S-transferase (GST) pull-down assays. These results demonstrate the robustness of the PPI screening system and provide a basis for studying the role of SNAPIN in regulating IAV replication.

Keywords
Influenza A virus; M1; NanoBiT; SNAPIN; protein-protein interaction.
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