STUB1-induced polyubiquitination of SIK3 in alveolar type 2 epithelial cells alleviates severity and outcomes of acute lung injury

Disruption in alveolar type 2 epithelial cells (AT2s) homeostasis by oxidative stress plays an essential role in the pathogenesis of acute lung injury (ALI). However, significant discrepancies remain in understanding the mechanisms for AT2 as a main target for Reactive Oxygen Species (ROS). Herein, we show that STUB1, an E3 Ligase involved in protein homeostasis, was dominantly expressed in AT2s. Mild levels of ROS potentiated Nrf2-mediated transactivation of the STUB1 gene via activation of the ERK signaling, whereas high levels of ROS compromised STUB1 expression by dampening STUB1 protein half-life. Ablation of Stub1 in AT2s caused failure in conferring K63-mediated nonproteolytic polyubiquitination of SIK3 (salt-inducible kinase 3), which in turn abrogated CRTC2 (CREB-regulated transcription co-activator 2) substrate binding for SIK3 and thereby triggered CREB signaling-mediated proinflammatory phenotypes. Consequently, disruption in STUB1/SIK3 signaling aggravated lung edema, augmented inflammatory infiltrate, and increased AT2 Apoptosis in vivo. Mice lacking STUB1 also demonstrated increased susceptibility to ischemia-reperfusion and overventilation-induced lung injury. These findings unambiguously uncover STUB1 as a critical post-translational regulator of ALI severity and outcomes.