Lignans Associated Differences in Salt Stress Responses of Flax (Linum usitatissimum L.) Genotypes In Vitro

  • Cells. 2026 Apr 28;15(9):796. doi: 10.3390/cells15090796.
Moumita Roy Chowdhury  1 ,  Katarína Ražná  1 ,  Jindra Valentová  2 ,  Emil Švajdlenka  2 ,  Eva Ivanišová  3 ,  Anirban Jyoti Debnath  4 ,  Jit Mukherjee  5 ,  Veronika Štefúnová  1 ,  Mizgin Mehmet  6 ,  Paračková Patrícia  2 ,  Marián Miko  1
Affiliations
  • 1. Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia.
  • 2. Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia.
  • 3. Institute of Food Sciences, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia.
  • 4. Institute of Chemistry and Environmental Science, Faculty of Natural Sciences, University of Ss. Cyril and Methodius in Trnava, Námestie Jozefa Herdu 2, 917 01 Trnava, Slovakia.
  • 5. Department of Computer Science and Engineering, Birla Institute of Technology, Mesra 835215, India.
  • 6. Department of Field Crops, Faculty of Agriculture, Ege University, Bornova, 35100 Izmir, Türkiye.
PMID: 42121897 DOI: 10.3390/cells15090796
Abstract

The objective of this study was to investigate the association between lignan content and stress responses in flax genotypes with contrasting lignan levels. For this purpose, two flax (Linum usitatissimum L.) genotypes, Agram and CDC Bethune, were selected based on their differing lignan profiles. We quantified secoisolariciresinol diglucoside, pinoresinol, pinoresinol diglucoside, matairesinol, and lariciresinol in both control and salt-stressed Plants. In parallel, antioxidant activity, flavonoid, Polyphenols, and phenolic acid content were determined to assess the overall antioxidant potential and phenolic response under saline conditions. The Agram genotype appears to activate defense mechanisms that enhance antioxidant capacity, which is largely mediated by polyphenolic compounds and distinct patterns of MicroRNA regulation. By contrast, the CDC Bethune genotype primarily responds to salinity stress by inducing lignan biosynthesis. Differential lignan modulation, contrasting Antioxidants and miRNA profiles, shows substantial intergenotypic differences in how flax activates distinct defense pathways.

Keywords
antioxidant analysis; elicitation in vitro; flax; lignan; microRNA; salt stress.
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