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BCAT2 Protein, Human (His, StrepII)

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Various studies have demonstrated that the BCAT2 protein plays a critical role in cellular processes as it catalyzes the initial reactions in the catabolism of the essential branched-chain amino acids leucine, isoleucine, and valine. This enzyme activity is essential for the breakdown of these amino acids, contributing to metabolic processes necessary for energy production and nitrogen balance. BCAT2 Protein, Human (His, Strep) is the recombinant human-derived BCAT2 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Various studies have demonstrated that the BCAT2 protein plays a critical role in cellular processes as it catalyzes the initial reactions in the catabolism of the essential branched-chain amino acids leucine, isoleucine, and valine. This enzyme activity is essential for the breakdown of these amino acids, contributing to metabolic processes necessary for energy production and nitrogen balance. BCAT2 Protein, Human (His, Strep) is the recombinant human-derived BCAT2 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.

Background

BCAT2 (Branched-chain amino acid transaminase 2) plays a key role in the catabolism of the essential branched-chain amino acids leucine, isoleucine, and valine. It catalyzes the first reaction in this process, involving the reversible transamination of these amino acids to their respective branched-chain alpha-keto acids. This enzymatic activity is crucial for regulating the levels of branched-chain amino acids and their metabolites, which are involved in various physiological processes, including energy metabolism and protein synthesis. Additionally, BCAT2 may function as a transporter for branched-chain alpha-keto acids, contributing to their cellular transport. It has to succinctly outline BCAT2's central role in branched-chain amino acid catabolism and suggests its potential involvement in the intracellular transport of related metabolites.

Verified Bioactivity

Measured by its ability to convert 2 mM leucine and 0.5 mM alpha-ketoglutarate to alpha-ketoisocaproate and glutamate at room temperature in kinetic mode for 8 minutes. The specific activity is 1920.85 pmol/min/μg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-StrepⅡ;N-6*His
  • Accession

    O15382 (A28-V392)

  • Gene ID
    587
  • Molecular Construction
    • N-term
    • StrepⅡ-6*His
    • BCAT2 (A28-V392)
      Accession # O15382
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    BCAT2; PP18; Prev. BCT2; Branched-Chain-Amino-Acid Aminotransferase; BCATm; Branched-Chain-Amino-Acid Transaminase; BCAM; BCAT(M); Branched-Chain-Amino-Acid Aminotransferase, Mitochondrial; ECA40; Branched Chain Aminotransferase 2, Mitochondrial; BCATM; P

  • AA Sequence

    ASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

  • Predicted Molecular Mass

    44.4 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 1 mM DTT, 20% glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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