CD3D-CD3E Heterodimer Protein, Human (Biotinylated, HEK293, hFc, His, Avi, Flag)

Measured by its binding ability in a functional ELISA. Immobilized Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His,Avitag&Fc,Flag,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a with a linear range of 0.4-3 ng/mL.

Human

HEK293

C-Avi;C-His;C-Flag;C-hFc

P04234-1 (F22-A105)&P07766 (D23-D126)

915&916

Biotin

The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH 7.4 with 10% trehalose as protectant.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.