CLDN1 Protein, Human (Cell-Free, His)
The CLDN1 protein is a tight junction component that regulates epithelial permeability. CLDN1 Protein, Human (Cell-Free, His) is the recombinant human-derived CLDN1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The CLDN1 protein is a tight junction component that regulates epithelial permeability. CLDN1 Protein, Human (Cell-Free, His) is the recombinant human-derived CLDN1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
CLDN1 protein, a member of the claudin family, plays a crucial role as a major constituent of tight junction complexes that regulate the permeability of epithelial barriers. While certain claudin family members contribute to the formation of impermeable barriers, others modulate the permeability to ions and small molecules. The interaction among various claudin family members, often coexpressed, determines the overall permeability of tight junctions. CLDN1 is specifically essential in preventing the paracellular diffusion of small molecules through tight junctions in the epidermis, crucial for maintaining the normal barrier function of the skin and ensuring water homeostasis. Interestingly, while CLDN1 appears dispensable for water barrier formation in keratinocyte tight junctions, it indirectly influences water loss, potentially through the regulation of other protein expression levels. Moreover, CLDN1 acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes, forming a receptor complex with CD81 that is vital for HCV entry into host cells, and it also functions as a receptor for dengue virus. These diverse roles underscore the significance of CLDN1 in both physiological barrier functions and viral infection processes.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-10*His
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Accession
O95832 (M1-V211)
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Gene ID9076
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Molecular Construction
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N-term
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10*His
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CLDN1 (M1-V211)
Accession # O95832 -
C-term
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Protein Length
Full Length
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Synonyms
CLDN1; Claudin-1; Claudin 1; CLD1; SEMP1; Senescence-Associated Epithelial Membrane Protein; ILVASC; Claudin; Senescence-Associated Epithelial Membrane Protein 1
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AA Sequence
MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
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Predicted Molecular Mass
24.2 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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