CSF1R Protein, Rat (HEK293, C-His)

CSF1R Protein is a cell surface receptor with high a affinity for a variety of polypeptide growth factors, cytokines, and hormones. CSF1R Protein participate in PLCG2/PKA/UCP2 signaling pathway to reduce oxidative stress and neuronal apoptosis in rat models with neonatal HIE. The blocking of the CSF1R signal is associated with tumorigenesis. CSF1R Protein, Rat (HEK293, C-His) is the recombinant rat-derived CSF1R, expressed by HEK293, with C-10*His labeled tag. The total length of CSF1R Protein, Rat (HEK293, C-His) is 491 a.a..

Receptor protein-tyrosine kinase is a cell surface receptor with a high affinity for a variety of polypeptide growth factors, cytokines, and hormones. Homologous with human CSF1R (colony-stimulating factor 1 receptor). CSF1R has macrophage colony-stimulating factor receptor activity, protein homodimerization activity and protein phosphatase binding activity. CSF1R is involved in the positive regulation of osteoclast differentiation and bone resorption. CSF1R participates in PLCG2/PKA/UCP2 signaling pathway to reduce oxidative stress and neuronal apoptosis in rat models with neonatal HIE. The blocking of the CSF1R signal can inhibit tumor-infiltrating myeloid cells and improve the therapeutic effect of radiotherapy for prostate cancer. In INS-1E beta-cells, Erk1/2 phosphorylation is activated by construction of the CSF1R/IRR receptor^[1][2][3][4].

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit the M-CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.2793 µg/mL in the presence of 10 ng/mL mouse M-CSF, corresponding to a specific activity is 3.58×10³ units/mg.

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  Measured by its ability to inhibit the M-CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.2793 µg/mL in the presence of 10 ng/mL mouse M-CSF, corresponding to a specific activity is 3.58×10³ units/mg.

Rat

HEK293

C-10*His

D4ACA7 (A64-E554)

307403

Partial

APVIEPSGPELVVEPGATVTLRCVSNGSVEWDGPISPYWTLDSESPGSILITKNATFKNTGTYRCTELEDPMRGSTAIHLYVKDPVRPWNLLAQEVTVFEGQEAVLPCLITDPTLKDSVSLVREWGRPVSRKTVYSFLPWRGFIIHKAKFLDSHTYMCKAVVNARESTSIGIRLKVNRAHPGPPHIILEPTKLVRIRGEAAQIVCSATHSEVEFNVILKRGDTKLEIPINSDFQDNAYKKVLTLNLNAVDFQDAGIYSCVANNAAGSNTATMNFQVVESAYLNLTSEQSLLQEVSVGENLDLTVIADAYPGLQRYNWTYLGPFFEDPHNLEFRTQWTTYSYSFKLHLNRVKPLEAGRYSLMAQNKAGWNNLTFELTLRYPPEVSVTWIPVNGSDVLLCDVSGYPQPNVTWMECRGHTDRCDEAQASQVWDDTQPEVLSQKPFHRVILQSQLPIGTLKHNMTYVCRAHNNVGNSSQFFRAISLGQSKQLPDE

Approximately 75-100 kDa due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zwick E, et al. Receptor tyrosine kinase signalling as a target for cancer intervention strategies. Endocr Relat Cancer. 2001 Sep;8(3):161-73.  [Content Brief]

  [2]. Hu X,et al. Rh-CSF1 Attenuates Oxidative Stress and Neuronal Apoptosis via the CSF1R/PLCG2/PKA/UCP2 Signaling Pathway in a Rat Model of Neonatal HIE. Oxid Med Cell Longev. 2020 Oct 7;2020:6801587.  [Content Brief]

  [3]. Xu J, et al. CSF1R signaling blockade stanches tumor-infiltrating myeloid cells and improves the efficacy of radiotherapy in prostate cancer. Cancer Res. 2013 May 1;73(9):2782-94.  [Content Brief]

  [4]. Keshvari S, et al. CSF1R-dependent macrophages control postnatal somatic growth and organ maturation. PLoS Genet. 2021 Jun 3;17(6):e1009605.  [Content Brief]