EGFR Protein, Mouse (HEK293, Fc)

The EGFR protein is a receptor tyrosine kinase that binds EGF family ligands and activates multiple signaling cascades. Ligand binding induces receptor homo- or heterodimerization, leading to autophosphorylation and recruitment of adapter proteins (such as GRB2). EGFR Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived EGFR protein, expressed by HEK293 , with C-hFc labeled tag. The total length of EGFR Protein, Mouse (HEK293, Fc) is 623 a.a., with molecular weight of ~142.57 kDa.

EGFR Protein, a receptor tyrosine kinase, binds ligands of the EGF family, such as EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG, and HBEGF/heparin-binding EGF, activating multiple signaling cascades to transduce extracellular cues into cellular responses. Ligand binding induces receptor homo- and/or heterodimerization, leading to autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2, initiating downstream signaling cascades, including RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs modules. EGFR may also activate the NF-kappa-B signaling cascade and directly phosphorylate proteins like RGS16, modulating G protein-coupled receptor signaling. Additionally, EGFR phosphorylates MUC1, enhancing its interaction with SRC and CTNNB1/beta-catenin. Notably, it positively regulates cell migration through interaction with CCDC88A/GIV, promoting EGFR signaling and triggering cell migration. EGFR also plays a role in enhancing learning and memory performance and contributes to mammalian pain signaling, leading to long-lasting hypersensitivity.

Immobilized Mouse EGFR, at 1 μg/mL (100 μL/well) can bind Anti- EGFR Antibody. The ED₅₀ is 0.7847 ng/mL, corresponding to a specific activity is 1.27×10⁶ U/mg.

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  Immobilized Mouse EGFR, at 1 μg/mL (100 μL/well) can bind Anti- EGFR Antibody. The ED50 is 0.7847 ng/mL, corresponding to a specific activity is 1.27×10⁶ U/mg.

Mouse

HEK293

C-hFc

Q01279 (L25-S647)

13649  [NCBI]

LEEKKVCQGTSNRLTQLGTFEDHFLSLQRMYNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAILSNYGTNRTGLRELPMRNLQEILIGAVRFSNNPILCNMDTIQWRDIVQNVFMSNMSMDLQSHPSSCPKCDPSCPNGSCWGGGEENCQKLTKIICAQQCSHRCRGRSPSDCCHNQCAAGCTGPRESDCLVCQKFQDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGIRKCKKCDGPCRKVCNGIGIGEFKDTLSINATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPDNWTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKIMNNRAEKDCKAVNHVCNPLCSSEGCWGPEPRDCVSCQNVSRGRECVEKCNILEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGIMGENNTLVWKYADANNVCHLCHANCTYGCAGPGLQGCEVWPSGPKIPS

Approximately 142.57 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.