EIF4A1 Protein, Human (His, StrepII)
Based on 1 Customer Validation
The EIF4A1 protein is an ATP-dependent RNA helicase and an important subunit of the eIF4F complex, which is essential for cap recognition and promoting the binding of mRNA to ribosomes. In the translation initiation model, EIF4A1 helps unravel RNA secondary structure in the mRNA 5'-UTR. EIF4A1 Protein, Human (His, Strep) is the recombinant human-derived EIF4A1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The EIF4A1 protein is an ATP-dependent RNA helicase and an important subunit of the eIF4F complex, which is essential for cap recognition and promoting the binding of mRNA to ribosomes. In the translation initiation model, EIF4A1 helps unravel RNA secondary structure in the mRNA 5'-UTR. EIF4A1 Protein, Human (His, Strep) is the recombinant human-derived EIF4A1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag.
EIF4A2, an ATP-dependent RNA helicase, functions as a crucial subunit of the eIF4F complex, playing a key role in cap recognition and facilitating mRNA binding to the ribosome. In the prevailing model of translation initiation, EIF4A2 is instrumental in unwinding RNA secondary structures present in the 5'-UTR of mRNAs. This unwinding activity is essential to enable the efficient binding of the small ribosomal subunit and subsequent scanning for the initiator codon, a fundamental process in the initiation of translation.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-StrepⅡ;N-6*His
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Accession
P60842 (M1-I406)
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Gene ID1973
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Molecular Construction
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N-term
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StrepⅡ-6*His
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EIF4A1 (M1-I406)
Accession # P60842 -
C-term
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Protein Length
Full Length
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Synonyms
EIF4A1; Eukaryotic Initiation Factor 4AI; Prev. EIF4A; EIF4A-I; DDX2A; Eukaryotic Translation Initiation Factor 4A, Isoform 1; EIF-4A; Eukaryotic Translation Initiation Factor 4A; ATP-Dependent RNA Helicase EIF4A-1; ATP-Dependent RNA Helicase; Eukaryotic
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AA Sequence
MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI
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Predicted Molecular Mass
49.3 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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