ESAM Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
ESAM protein promotes aggregation through a possible homophilic molecular interaction and interacts with MAGI1. ESAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived ESAM protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ESAM protein promotes aggregation through a possible homophilic molecular interaction and interacts with MAGI1. ESAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived ESAM protein, expressed by HEK293 , with C-His labeled tag.
Background
ESAM protein is capable of facilitating aggregation, likely through a homophilic molecular interaction. It has been observed to interact with MAGI1.
Verified Bioactivity
Measured by its ability to chemoattract Jurkat Human T lymphocyte leukemia cells. The ED50 for this effect is 137.6 ng/mL, corresponding to a specific activity is 7.267×103 U/mg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
Q925F2 (Q30-A251)
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Molecular Construction
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N-term
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ESAM (Q30-A251)
Accession # Q925F2 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ESAM; HUEL (C4orf1)-Interacting Protein; Endothelial Cell Adhesion Molecule; LP4791 Protein; Endothelial Cell-Selective Adhesion Molecule; 2310008D05Rik; W117m; NEDIHSS
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AA Sequence
QMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESWSHPREVPILIWFLEQEGKEPNQVLSYINGVMTNKPGTALVHSISSRNVSLRLGALQEGDSGTYRCSVNVQNDEGKSIGHSIKSIELKVLVPPAPPSCSLQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDAVRGSLKLTNLSIAMSGVYVCKAQNRVGFAKCNVTLDVMTGSKAA
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Molecular Weight
Approximately 33-43 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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