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Ferritin heavy chain/FTH1 Protein, Human

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Based on 1 publication(s) in Google Scholar

The ferritin heavy chain/FTH1 protein is critical for maintaining iron homeostasis, storing iron in soluble form through ferroxidase activity. It promotes the oxidation of ferrous iron so that it can be deposited in the form of iron hydroxide. Ferritin heavy chain/FTH1 Protein, Human is the recombinant human-derived Ferritin heavy chain/FTH1 protein, expressed by E. coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Ferritin heavy chain/FTH1 Protein, Human
  • Experimental Validation Results for Ferritin heavy chain/FTH1 Protein, Human
  • Ferritin heavy chain/FTH1 Protein, Human Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The ferritin heavy chain/FTH1 protein is critical for maintaining iron homeostasis, storing iron in soluble form through ferroxidase activity. It promotes the oxidation of ferrous iron so that it can be deposited in the form of iron hydroxide. Ferritin heavy chain/FTH1 Protein, Human is the recombinant human-derived Ferritin heavy chain/FTH1 protein, expressed by E. coli , with tag free.

Background

Ferritin heavy chain/FTH1 protein serves a crucial role in iron homeostasis by storing iron in a soluble and non-toxic form. With ferroxidase activity, it facilitates the oxidation of ferrous iron, allowing its deposition as ferric hydroxides. Beyond iron storage, this protein also contributes to the delivery of iron to cells, emphasizing its multifaceted function. Moreover, FTH1 is implicated in mediating iron uptake in capsule cells during kidney development, further highlighting its significance in various physiological processes.

Verified Bioactivity

Immobilized Human FTH1 at 2 μg/mL (100 μL/well) can bind FTH1 antibody .The ED50 for this effect is 1-5 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Human FTH1 at 2 μg/mL (100 μL/well) can bind FTH1 antibody .The ED50 for this effect is 2.771 ng/mL.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P02794 (M1-S183)

  • Gene ID
    2495  [NCBI]
  • Molecular Construction
    • N-term
    • FTH1 (M1-S183)
      Accession # P02794
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    FTH1; Proliferation-Inducing Protein 15; Prev. FTHL6; Ferritin, Heavy Polypeptide 1; FTH; Ferritin H Subunit; Ferritin Heavy Chain; Apoferritin; PIG15; H-Ferritin; PLIF; Ferritin; FHC; NBIA9; Cell Proliferation-Inducing Gene 15 Protein; HFE5; Ferritin Heavy Chain 1; Placenta Immunoregulatory Factor

  • AA Sequence

    MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES

  • Predicted Molecular Mass

    21.2 kDa

  • Molecular Weight

    Approximately 20-22 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Human FTH1 at 2 μg/mL (100 μL/well) can bind FTH1 antibody .The ED50 for this effect is 2.771 ng/mL.

  • Ferritin heavy chain/FTH1 Protein, Human Product Image
    1/16
  • Experimental Validation Results for Ferritin heavy chain/FTH1 Protein, Human
  • Experimental Validation Results for Ferritin heavy chain/FTH1 Protein, Human
  • Ferritin heavy chain/FTH1 Protein, Human Product Image