FIP1L1 Protein, Human (Sf9, His, GST)

The FIP1L1 protein is an important component of the CPSF complex and is responsible for the formation of the 3' end of pre-mRNA. FIP1L1 Protein, Human (Sf9, His, GST) is the recombinant human-derived FIP1L1 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of FIP1L1 Protein, Human (Sf9, His, GST) is 593 a.a., .

FIP1L1, a vital component of the cleavage and polyadenylation specificity factor (CPSF) complex, plays a crucial role in pre-mRNA 3'-end formation by recognizing the AAUAAA signal sequence and facilitating interactions with poly(A) polymerase and other factors, leading to cleavage and poly(A) addition. Notably, FIP1L1 contributes to poly(A) site recognition, stimulates poly(A) addition, and binds to U-rich RNA sequence elements surrounding the poly(A) site. Its presence in the CPSF complex, alongside CPSF1, CPSF2, CPSF3, and CPSF4, highlights its integral role in the intricate process of pre-mRNA processing. FIP1L1 forms specific interactions with various partners, including CPSF1, CPSF4, CSTF2, and CSTF3, influencing the overall functionality of the CPSF complex. Moreover, FIP1L1 engages in direct interactions with AHCYL1 when phosphorylated, linking AHCYL1 to the CPSF complex and RNA. Additionally, it interacts with NUDT21/CPSF5 in a sequence-specific manner. Furthermore, FIP1L1 engages in interactions with CPSF6 and CPSF7, mediated by specific domains, contributing to the coordination between the CFIm and CPSF complexes. The interaction with PAPOLA is also noted, potentially modulated by its association with AHCYL1 (By similarity).

Human

Sf9 insect cells

N-8*His;N-GST

Q6UN15 (S2-E594)

81608

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.