Fractalkine/CX3CL1 Protein, Mouse (HEK293, His)
Based on 2 publication(s) in Google Scholar
The Fractalkine/CX3CL1 protein is a multifunctional chemokine that binds to CX3CR1 and the integrins ITGAV:ITGB3 and ITGA4:ITGB1.It regulates immune responses, inflammation, cell adhesion, and chemotaxis.Fractalkine/CX3CL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
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Biological Activity
Description
The Fractalkine/CX3CL1 protein is a multifunctional chemokine that binds to CX3CR1 and the integrins ITGAV:ITGB3 and ITGA4:ITGB1.It regulates immune responses, inflammation, cell adhesion, and chemotaxis.Fractalkine/CX3CL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Fractalkine/CX3CL1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Fractalkine/CX3CL1 protein functions as a versatile chemokine, serving as a ligand for both CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. The CX3CR1-CX3CL1 signaling axis manifests distinct functions in various tissue compartments, including immune response modulation, inflammation regulation, cell adhesion, and chemotaxis. In the context of endothelial interactions, Fractalkine/CX3CL1 plays a pivotal role in regulating leukocyte adhesion and migration processes. Notably, it can activate integrins in a CX3CR1-dependent or CX3CR1-independent manner, binding to both the classical ligand-binding site (site 1) and a distinct site (site 2) on integrins. In the presence of CX3CR1, it activates integrins directly through site 1, while in the absence of CX3CR1, it binds to site 2, enhancing the binding of other integrin ligands to site 1. Additionally, the soluble form of Fractalkine/CX3CL1 demonstrates chemotactic properties for T-cells and monocytes, albeit not for neutrophils.
Verified Bioactivity
Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50 this effect is 9.640 ng/mL, corresponding to a specific activity is 103734.440 U/mg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Oncol (Dordr)
CX3CL1-CX3CR1 signaling orchestrates malignant progression of prostate cancer through luminal progenitor-macrophage crosstalk. [Abstract]2026 Mar 23;49(2):59. PMID: 41870750 -
Cell Signal
CX3CL1 represses autophagy via CX3CR1/ CaMKIIδ/HDAC4/Rubicon axis and exacerbates chronic intermittent hypoxia induced Kupffer cell apoptosis. [Abstract]2023 Nov:111:110873. PMID: 37640194
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
O35188 (Q25-R337)
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Molecular Construction
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N-term
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Fractalkine (Q25-R337)
Accession # O35188 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CX3CL1; NTN; Prev. SCYD1; CX3C Membrane-Anchored Chemokine; Chemokine (C-X3-C Motif) Ligand 1; Small-Inducible Cytokine D1; Fractalkine; C-X3-C Motif Chemokine 1; Neurotactin; NTT; C3Xkine; Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member-1; ABCD
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AA Sequence
QHLGMTKCEIMCDKMTSRIPVALLIRYQLNQESCGKRAIVLETTQHRRFCADPKEKWVQDAMKHLDHQAAALTKNGGKFEKRVDNVTPGITLATRGLSPSALTKPESATLEDLALELTTISQEARGTMGTSQEPPAAVTGSSLSTSEAQDAGLTAKPQSIGSFEAADISTTVWPSPAVYQSGSSSWAEEKATESPSTTAPSPQVSTTSPSTPEENVGSEGQPPWVQGQDLSPEKSLGSEEINPVHTDNFQERGPGNTVHPSVAPISSEETPSPELVASGSQAPKIEEPIHATADPQKLSVLITPVPDTQAATR
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Predicted Molecular Mass
33.2 kDa
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Molecular Weight
Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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