Gremlin-1 Protein, Rat (sf9, His, Myc)
Based on 1 Customer Validation
Gremlin-1 Protein, Rat (sf9, His, Myc) is the recombinant human-derived Gremlin-1 protein, expressed by sf9 insect cells.
- Species: Rat
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Gremlin-1 Protein, Rat (sf9, His, Myc) is the recombinant human-derived Gremlin-1 protein, expressed by sf9 insect cells.
Background
Gremlin-1 is a cytokine potentially involved in carcinogenesis and metanephric kidney organogenesis. Functioning as a BMP antagonist, it is essential for early limb outgrowth and patterning by maintaining the FGF4-SHH feedback loop. It downregulates BMP4 signaling in a dose-dependent manner (By similarity). As an antagonist of BMP2, it inhibits BMP2-mediated osteoblast differentiation (in vitro). Additionally, it acts as an inhibitor of monocyte chemotaxis. Overexpression can suppress the growth/viability of normal cells but not transformed cells (By similarity).
Technical Parameters
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Species Rat
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Source Sf9 insect cells
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Tag N-10*His;C-Myc
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Accession
O35793 (K25-D184)
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Gene ID50566
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Molecular Construction
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N-term
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10*His
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Gremlin-1 (K25-D184)
Accession # O35793 -
Myc
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
GREM1; IHG-2; Prev. CKTSF1B1; Gremlin 1, Cysteine Knot Superfamily, Homolog; Prev. CRAC1; Down-Regulated In Mos-Transformed Cells; Gremlin; Increased In High Glucose Protein 2; DAND2; Increased In High Glucose-2; DRM; Gremlin 1-Like Protein; Cysteine Knot
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AA Sequence
KKKGSQGAIPPPDKAQHNDSEQTQSPPQPGSRTRGRGQGRGTAMPGEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSFQSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD
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Predicted Molecular Mass
22.2 kDa
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Molecular Weight
Approximately 29 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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