HIST3H2A Protein, Human
Based on 1 Customer Validation
The HIST3H2A protein is a core component of nucleosomes that compact DNA into chromatin. HIST3H2A Protein, Human is the recombinant human-derived HIST3H2A protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The HIST3H2A protein is a core component of nucleosomes that compact DNA into chromatin. HIST3H2A Protein, Human is the recombinant human-derived HIST3H2A protein, expressed by E. coli , with tag free.
HIST3H2A, as a core component of the nucleosome, participates in the essential process of wrapping and compacting DNA into chromatin, thereby limiting DNA accessibility to cellular machineries that rely on DNA as a template. This histone protein, like others, holds a pivotal position in key cellular functions such as transcription regulation, DNA repair, DNA replication, and the maintenance of chromosomal stability. The intricate control of DNA accessibility involves a sophisticated system of post-translational modifications known as the histone code, along with dynamic nucleosome remodeling. The nucleosome itself is a histone octamer comprising two molecules each of H2A, H2B, H3, and H4, assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. This octamer efficiently wraps approximately 147 base pairs of DNA, underscoring its crucial role in organizing chromatin structure and facilitating essential genomic processes.
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
Q7L7L0 (S2-K130)
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Gene ID92815 [NCBI]
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Molecular Construction
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N-term
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HIST3H2A (S2-K130)
Accession # Q7L7L0 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
H2AC25; Histone H2A Type 3; Prev. HIST3H2A; H2A.W Histone; Prev. H2AW; MGC3165; Histone Cluster 3, H2a; H2A-Clustered Histone 25; Histone Cluster 3 H2A; H2A Clustered Histone 25; Histone 3, H2a
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AA Sequence
SGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK
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Predicted Molecular Mass
14 kDa
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Molecular Weight
Approximately 15-20 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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