IDH1 Protein, Human (His)
Based on 1 Customer Validation
The IDH1 protein catalyzes the oxidative decarboxylation of isocitrate to generate 2-oxoglutarate, which is utilized by enzymes such as phytoacyl-CoA dioxygenase. IDH1 Protein, Human (His) is the recombinant human-derived IDH1 protein, expressed by E. coli, with C-8*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The IDH1 protein catalyzes the oxidative decarboxylation of isocitrate to generate 2-oxoglutarate, which is utilized by enzymes such as phytoacyl-CoA dioxygenase. IDH1 Protein, Human (His) is the recombinant human-derived IDH1 protein, expressed by E. coli, with C-8*His labeled tag.
IDH1 protein is a key enzyme that catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), a vital step in cellular metabolism and a process required by other enzymes such as the phytanoyl-CoA dioxygenase. The enzymatic activity of IDH1 is crucial for the generation of NADPH, a critical cofactor involved in various biosynthesis pathways. Additionally, IDH1 may have a distinct role as a corneal epithelial crystallin, potentially contributing to the maintenance of corneal epithelial transparency. These multifaceted functions highlight the importance of IDH1 in fundamental cellular processes and suggest its involvement in maintaining specific tissue characteristics.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-8*His
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Accession
O75874 (M1-L414)
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Gene ID3417
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Molecular Construction
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N-term
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IDH1 (M1-L414)
Accession # O75874 -
8*His
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C-term
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Protein Length
Full Length
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Synonyms
IDH1; NADP+-Specific Isocitrate Dehydrogenase; Isocitrate Dehydrogenase (NADP(+)) 1; Isocitrate Dehydrogenase 1 (NADP+); Oxalosuccinate Decarboxylase; Epididymis Secretory Protein Li 26; NADP(+)-Specific ICDH; Isocitrate Dehydrogenase (NADP(+)); Isocitrat
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AA Sequence
MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVGVKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNKELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL
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Predicted Molecular Mass
48.1 kDa
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Molecular Weight
Approximately 39-54 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50mM Tris-HCl, 200mM NaCl, 10% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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