IGFBP-1 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
The IGFBP-1 protein is an insulin-like growth factor binding protein that extends the half-life of IGF and modulates its growth-promoting effects in cell cultures, thereby inhibiting or stimulating these responses. Crucially, it alters the interaction between IGF and its cell surface receptors and promotes cell migration. IGFBP-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-1 protein, expressed by HEK293 , with C-10*His tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The IGFBP-1 protein is an insulin-like growth factor binding protein that extends the half-life of IGF and modulates its growth-promoting effects in cell cultures, thereby inhibiting or stimulating these responses. Crucially, it alters the interaction between IGF and its cell surface receptors and promotes cell migration. IGFBP-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-1 protein, expressed by HEK293 , with C-10*His tag.
IGFBP-1, an insulin-like growth factor-binding protein, contributes to the regulation of IGFs by extending their half-life and modulating their growth-promoting effects on cell culture, either inhibiting or stimulating these effects. This protein plays a crucial role in altering the interaction between IGFs and their cell surface receptors. Additionally, IGFBP-1 demonstrates the ability to promote cell migration, indicating its involvement in cellular processes beyond growth regulation. Notably, it exhibits equal binding affinity for both IGF1 and IGF2, highlighting its versatile role in modulating the biological activities of insulin-like growth factors.
Measured by its ability to inhibit the biological activity of IGF-I on MCF 7 human breast cancer cells. The ED50 this effect is <1.5 µg/mL in the presence of 2.5 ng/mL IGF-I.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
P47876 (A26-N272)
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Gene ID16006
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Molecular Construction
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N-term
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IGFBP-1 (A26-N272)
Accession # P47876 -
10*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IGFBP1; Alpha-Pregnancy-Associated Endometrial Globulin; Prev. IBP1; Amniotic Fluid Binding Protein; PP12; Binding Protein-28; Insulin-Like Growth Factor-Binding Protein 1; Binding Protein-26; IGF-Binding Protein 1; Binding Protein-25; Placental Protein 1
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AA Sequence
APQPWHCAPCTAERLGLCPPVPASCPEISRPAGCGCCPTCALPMGAACGVATARCAQGLSCRALPGEPRPLHALTRGQGACVPEPAAPATSTLFSSQHEEAKAAVVSADELSESPEMTEEQLLDSFHLMAPSREDQPILWNAISTYSSMRAREIADLKKWKEPCQRELYKVLERLAAAQQKAGDEIYKFYLPNCNKNGFYHSKQCETSLDGEAGLCWCVYPWSGKKIPGSLETRGDPNCHQYFNVHN
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Predicted Molecular Mass
28.2 kDa
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Molecular Weight
Approximately 33-34 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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