IL-18 Protein, Rhesus Macaque (His)

IL-18 protein is a pro-inflammatory cytokine that is critically involved in epithelial barrier repair and regulates immune responses, especially Th1 cell and NK cell immune responses. IL-18 binds to IL18R1 and IL18RAP receptors to form a signaling ternary complex, activate NF-κ-B, and induce inflammatory mediators. IL-18 Protein, Rhesus Macaque (His) is the recombinant Rhesus Macaque-derived IL-18 protein, expressed by E. coli , with N-6*His, labeled tag.

IL-18 Protein is a pro-inflammatory cytokine that plays a crucial role in epithelial barrier repair and the modulation of immune responses, specifically in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to its receptors IL18R1 and IL18RAP, IL-18 forms a signaling ternary complex that activates NF-kappa-B, resulting in the synthesis of inflammatory mediators. It also works synergistically with IL-12/interleukin-12 to induce the synthesis of IFNG from Th1 cells and NK cells. Furthermore, IL-18 is involved in transducing inflammation downstream of pyroptosis, where its mature form is selectively released into the extracellular space through the gasdermin-D (GSDMD) pore.

Rhesus Macaque

E. coli

N-6*His;

Q9BGI5/NP_001028006.1 (A2-D193)

574151  [NCBI]

Full Length of Mature Protein (with Propeptide)

AAEPAEDNCINFVAMKFIDSTLYFIAEDDENLESDYFGKLESKLSIIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIINMYKDSQPRGMAVAISVKCEKISTLSCENRIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLYKLILKKKDELGDRSIMFTVQNED

Approximately 24 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.