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CSF1R Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CSF1R protein is ubiquitously found in mononuclear phagocytes, especially macrophages and monocytes, and is involved in a variety of biological processes. It induces the release of proinflammatory chemokines in response to IL34 and CSF1, contributing to innate immunity and inflammation. CSF1R Protein, Human (HEK293, His) is the recombinant human-derived CSF1R protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CSF1R Protein, Human (HEK293, His)
  • CSF1R Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

CSF1R protein is ubiquitously found in mononuclear phagocytes, especially macrophages and monocytes, and is involved in a variety of biological processes. It induces the release of proinflammatory chemokines in response to IL34 and CSF1, contributing to innate immunity and inflammation. CSF1R Protein, Human (HEK293, His) is the recombinant human-derived CSF1R protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The CSF1R protein, particularly found in mononuclear phagocytes like macrophages and monocytes, is involved in various biological processes. It promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, contributing to innate immunity and inflammatory processes. Additionally, CSF1R is crucial for regulating osteoclast proliferation and differentiation, bone resorption, normal bone and tooth development. It is also required for normal fertility in both males and females, as well as for the development of milk ducts and acinar structures in the mammary gland during pregnancy.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Human CSF1R at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF. The ED50 for this effect is 24.66 ng/mL.
3.Immobilized Recombinant Human M-CSF R (C-6His) at 5 μg/mL (100 μL/well) can bind Recombinant Human M-CSF. Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human M-CSF is 0.1-0.5 μg/mL.
4.Immobilized Recombinant Human M-CSF R (C-6His) at 10 μg/mL (100 μL/well) can bind Recombinant Human M-CSF. Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human M-CSF is 0.1-0.5 μg/mL.

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human CSF1R at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF. The ED50 for this effect is 24.66 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P07333-1/NP_005202.2 (I20-P517)

  • Gene ID
    1436  [NCBI]
  • Molecular Construction
    • N-term
    • CSF1R (I20-P517)
      Accession # P07333-1/NP_005202.2
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CSF1R; CSF-1R; Prev. FMS; Macrophage Colony Stimulating Factor I Receptor; C-FMS; Colony Stimulating Factor Receptor; CD115; Receptor Protein-Tyrosine Kinase; CSFR; FMS Proto-Oncogene; McDonough Feline Sarcoma Viral (V-Fms) Oncogene Homolog; BANDDOS; Macr

  • AA Sequence

    IPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLCAASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQSLLTVETLEHNQTYECRAHNSVGSGSWAFIPISAGAHTHPPDEFLFTP

  • Predicted Molecular Mass

    55.9 kDa

  • Molecular Weight

    Approximately 80-110 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human CSF1R at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF. The ED50 for this effect is 24.66 ng/mL.

  • CSF1R Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CSF1R Protein, Human (HEK293, His)
  • CSF1R Protein, Human (HEK293, His) Product Image