CSF1R Protein, Cynomolgus (HEK293, Fc)

The CSF1R protein is a tyrosine protein kinase receptor for CSF1 and IL34 and plays a critical regulatory role in hematopoietic cells, especially mononuclear phagocytes. Its effects span innate immunity, inflammation, osteoclast function, skeletal development, and fertility. CSF1R Protein, Cynomolgus (HEK293, Fc) is the recombinant cynomolgus-derived CSF1R protein, expressed by HEK293, with C-hFc labeled tag.

Colony-stimulating factor 1 receptor (CSF1R) is a type I transmembrane protein receptor and tyrosine kinase involved in cell survival, proliferation, and differentiation. Colony-stimulating factor-1 (CSF-1) and interleukin-34 (IL-34) are extracellular ligands of CSF1R. This endogenous cytokine activates the extracellular domain of CSF1R and triggers intracellular tyrosine kinases. Autophosphorylation of the domain subsequently activates multiple downstream pro-survival kinase cascades, including PI3K, ERK1/2, and JNK. The intracellular domain of CSF1R contains autophosphorylated tyrosine residues, which then recruit and complex with multiple downstream effector proteins/pathways, including PI3K, Grb2, SFK, Src, PKC, and others. CSF1R is ubiquitously expressed in macrophages, microglia, osteoclasts, and bone marrow dendritic cells but is rarely expressed in hematopoietic stem cells. Studies have found that CSF1R is involved in embryonic development and tissue repair, and is related to tumorigenesis and cancer progression. CSF1R can regulate tumor-associated macrophages (TAMs), causing TAMs to release tumorigenic cytokines, further dysregulated the tumor microenvironment and forming a pro-tumor phenotype. CSF1R mutations are also associated with neurodegenerative diseases and skeletal abnormalities.

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit the M-CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.003272 µg/mL in the presence of 1 ng/mL of Recombinant Human M-CSF, corresponding to a specific activity is 3.06×10⁵ units/mg.

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  Measured by its ability to inhibit the M-CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.003272 µg/mL in the presence of 1 ng/mL of Recombinant Human M-CSF, corresponding to a specific activity is 3.06×10⁵ units/mg.

Cynomolgus

HEK293

C-6*His

XP_005558297 (I99-P596)

102124214  [NCBI]

Partial

IPVIEPSGPELVVKPGETVTLRCVGNGSVEWDGPISPHWTLYSDGPSSVLTTNNATFQNTRTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAKEVVVFEDQDALLPCLLTDPVLEAGVSLVRLRGRPLLRHTNYSFSPWHGFIIHRAKFIQGQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASNIDVDFDVFLQHNTTKLAIPQRSDFHDNRYQKVLTLSLGQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLDLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTSINGSGTLLCAASGYPQPNVTWLQCAGHTDRCDEAQVLQVWVDPHPEVLSQEPFQKVTVQSLLTAETLEHNQTYECRAHNSVGSGSWAFIPISAGARTHPPDEFLFTP

80-120 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.